Antitumor Activity of an Immunotoxin in a Nude Mouse Model of Human Ovarian Cancer

D. J. Fitzgerald; M. J. Bjorn; R. J. Ferris; J. L. Winkelhake; A. E. Frankel; T. C. Hamilton; R. F. Ozols; M. C. Willingham; I. Pastan

Antitumor Activity of an Immunotoxin in a Nude Mouse Model of Human Ovarian Cancer

D. J. Fitzgerald, M. J. Bjorn, R. J. Ferris, J. L. Winkelhake, A. E. Frankel, T. C. Hamilton, R. F. Ozols, M. C. Willingham, I. Pastan

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Abstract

An immunotoxin composed of an antibody to the human transferrin receptor (454A12) and ricin A chain (RTA) was shown to inhibit the growth of NIH:OVCAR-3 tumors in a nude mouse model of human ovarian cancer. Inhibition of tumor growth by 454A12-RTA was related to the dose administered. The antitumor activity of the immunotoxin was blocked by coinjection of excess antibody with immunotoxin. An immunotoxin made using 454Al2 and recombinant ricin A chain (rRTA) had an activity similar to that made with native RTA. The administration of 10 μg or greater of the immunotoxin 454A12-RTA/rRTA had significant antitumor activity. The injection of 30 μg of an irrelevant immunotoxin, MOPC21-RTA, or 30 to 500 μg of the 454A12 antibody had no antitumor activity.

Original language	English (US)
Pages (from-to)	1407-1410
Number of pages	4
Journal	Cancer research
Volume	47
Issue number	5
State	Published - Mar 1987

ASJC Scopus subject areas

Oncology
Cancer Research

Cite this

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title = "Antitumor Activity of an Immunotoxin in a Nude Mouse Model of Human Ovarian Cancer",

abstract = "An immunotoxin composed of an antibody to the human transferrin receptor (454A12) and ricin A chain (RTA) was shown to inhibit the growth of NIH:OVCAR-3 tumors in a nude mouse model of human ovarian cancer. Inhibition of tumor growth by 454A12-RTA was related to the dose administered. The antitumor activity of the immunotoxin was blocked by coinjection of excess antibody with immunotoxin. An immunotoxin made using 454Al2 and recombinant ricin A chain (rRTA) had an activity similar to that made with native RTA. The administration of 10 μg or greater of the immunotoxin 454A12-RTA/rRTA had significant antitumor activity. The injection of 30 μg of an irrelevant immunotoxin, MOPC21-RTA, or 30 to 500 μg of the 454A12 antibody had no antitumor activity.",

author = "Fitzgerald, {D. J.} and Bjorn, {M. J.} and Ferris, {R. J.} and Winkelhake, {J. L.} and Frankel, {A. E.} and Hamilton, {T. C.} and Ozols, {R. F.} and Willingham, {M. C.} and I. Pastan",

year = "1987",

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AU - Fitzgerald, D. J.

AU - Bjorn, M. J.

AU - Ferris, R. J.

AU - Winkelhake, J. L.

AU - Frankel, A. E.

AU - Hamilton, T. C.

AU - Ozols, R. F.

AU - Willingham, M. C.

AU - Pastan, I.

PY - 1987/3

Y1 - 1987/3

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AB - An immunotoxin composed of an antibody to the human transferrin receptor (454A12) and ricin A chain (RTA) was shown to inhibit the growth of NIH:OVCAR-3 tumors in a nude mouse model of human ovarian cancer. Inhibition of tumor growth by 454A12-RTA was related to the dose administered. The antitumor activity of the immunotoxin was blocked by coinjection of excess antibody with immunotoxin. An immunotoxin made using 454Al2 and recombinant ricin A chain (rRTA) had an activity similar to that made with native RTA. The administration of 10 μg or greater of the immunotoxin 454A12-RTA/rRTA had significant antitumor activity. The injection of 30 μg of an irrelevant immunotoxin, MOPC21-RTA, or 30 to 500 μg of the 454A12 antibody had no antitumor activity.

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Antitumor Activity of an Immunotoxin in a Nude Mouse Model of Human Ovarian Cancer

Abstract

ASJC Scopus subject areas

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