Antitumor effect of snake venom (Hydrophis spiralis) on Ehrlich Ascites Carcinoma bearing mice

R. Karthikeyan, S. Karthigayan, M. Sri Balasubashini, S. Vijayalakshmi, S. T. Somasundaram, T. Balasubramanian

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The present study was carried out to assess the antitumor effect of venom from snake, Hydrophis spiralis on the Ehrlich Ascites Carcinoma (EAC). Four groups of albino Swiss mice were treated with three doses of the sub lethal dose of venom, viz., 0.418, 4.18 and 6.27 μg kg-1 body weight (intraperitoneal injection) along with the standard drug 5 flurouracil (20 mg kg-1 b.w.). The biochemical analysis and rest was left to calculate the mean survival time. In EAC bearing mice, mean life span tumor volume, hemoglobin, red blood cell and lymphocytes were significantly decreased when compared to the normal animals. Whereas, body weight neutrophils and viable tumor cell count was increased in the EAC bearing mice. These changes were brought back to near normal levels in different treatment groups. The macromolecule concentration of peritoneal cells, such as, DNA, RNA and protein, were altered in the EAC bearing mice and observed to be near normal in the treatment groups. The caspase 3 activity was significantly increased in the peritoneal cells of the treatment groups when compared to the EAC bearing mice. The role of apoptotic cascade in EAC cell death was confirmed by the DNA fragmentation on agarose gel. Apart from the antitumor effect, snake venom reduced the tumor burden on the liver and altered the changes in the activities of alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP). Hence the venom from H. Spiralis has a potential antitumor effect on the EAC bearing mice.

Original languageEnglish (US)
Pages (from-to)167-173
Number of pages7
JournalInternational Journal of Cancer Research
Volume3
Issue number4
StatePublished - Oct 25 2007

Fingerprint

Snake Venoms
Ascites
Carcinoma
Venoms
Tumor Burden
Body Weight
DNA Fragmentation
Aspartate Aminotransferases
Intraperitoneal Injections
Alanine Transaminase
Caspase 3
Sepharose
Alkaline Phosphatase
Hemoglobins
Neutrophils
Cell Death
Therapeutics
Cell Count
Erythrocytes
Gels

Keywords

  • Antitumor
  • Caspase 3
  • DNA fragmentation
  • EAC
  • Snake venom

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Karthikeyan, R., Karthigayan, S., Sri Balasubashini, M., Vijayalakshmi, S., Somasundaram, S. T., & Balasubramanian, T. (2007). Antitumor effect of snake venom (Hydrophis spiralis) on Ehrlich Ascites Carcinoma bearing mice. International Journal of Cancer Research, 3(4), 167-173.

Antitumor effect of snake venom (Hydrophis spiralis) on Ehrlich Ascites Carcinoma bearing mice. / Karthikeyan, R.; Karthigayan, S.; Sri Balasubashini, M.; Vijayalakshmi, S.; Somasundaram, S. T.; Balasubramanian, T.

In: International Journal of Cancer Research, Vol. 3, No. 4, 25.10.2007, p. 167-173.

Research output: Contribution to journalArticle

Karthikeyan, R, Karthigayan, S, Sri Balasubashini, M, Vijayalakshmi, S, Somasundaram, ST & Balasubramanian, T 2007, 'Antitumor effect of snake venom (Hydrophis spiralis) on Ehrlich Ascites Carcinoma bearing mice', International Journal of Cancer Research, vol. 3, no. 4, pp. 167-173.
Karthikeyan R, Karthigayan S, Sri Balasubashini M, Vijayalakshmi S, Somasundaram ST, Balasubramanian T. Antitumor effect of snake venom (Hydrophis spiralis) on Ehrlich Ascites Carcinoma bearing mice. International Journal of Cancer Research. 2007 Oct 25;3(4):167-173.
Karthikeyan, R. ; Karthigayan, S. ; Sri Balasubashini, M. ; Vijayalakshmi, S. ; Somasundaram, S. T. ; Balasubramanian, T. / Antitumor effect of snake venom (Hydrophis spiralis) on Ehrlich Ascites Carcinoma bearing mice. In: International Journal of Cancer Research. 2007 ; Vol. 3, No. 4. pp. 167-173.
@article{75a7d8a8739d46bfb86ceea30a077fdf,
title = "Antitumor effect of snake venom (Hydrophis spiralis) on Ehrlich Ascites Carcinoma bearing mice",
abstract = "The present study was carried out to assess the antitumor effect of venom from snake, Hydrophis spiralis on the Ehrlich Ascites Carcinoma (EAC). Four groups of albino Swiss mice were treated with three doses of the sub lethal dose of venom, viz., 0.418, 4.18 and 6.27 μg kg-1 body weight (intraperitoneal injection) along with the standard drug 5 flurouracil (20 mg kg-1 b.w.). The biochemical analysis and rest was left to calculate the mean survival time. In EAC bearing mice, mean life span tumor volume, hemoglobin, red blood cell and lymphocytes were significantly decreased when compared to the normal animals. Whereas, body weight neutrophils and viable tumor cell count was increased in the EAC bearing mice. These changes were brought back to near normal levels in different treatment groups. The macromolecule concentration of peritoneal cells, such as, DNA, RNA and protein, were altered in the EAC bearing mice and observed to be near normal in the treatment groups. The caspase 3 activity was significantly increased in the peritoneal cells of the treatment groups when compared to the EAC bearing mice. The role of apoptotic cascade in EAC cell death was confirmed by the DNA fragmentation on agarose gel. Apart from the antitumor effect, snake venom reduced the tumor burden on the liver and altered the changes in the activities of alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP). Hence the venom from H. Spiralis has a potential antitumor effect on the EAC bearing mice.",
keywords = "Antitumor, Caspase 3, DNA fragmentation, EAC, Snake venom",
author = "R. Karthikeyan and S. Karthigayan and {Sri Balasubashini}, M. and S. Vijayalakshmi and Somasundaram, {S. T.} and T. Balasubramanian",
year = "2007",
month = "10",
day = "25",
language = "English (US)",
volume = "3",
pages = "167--173",
journal = "International Journal of Cancer Research",
issn = "1811-9727",
publisher = "Asian Network for Scientific Information",
number = "4",

}

TY - JOUR

T1 - Antitumor effect of snake venom (Hydrophis spiralis) on Ehrlich Ascites Carcinoma bearing mice

AU - Karthikeyan, R.

AU - Karthigayan, S.

AU - Sri Balasubashini, M.

AU - Vijayalakshmi, S.

AU - Somasundaram, S. T.

AU - Balasubramanian, T.

PY - 2007/10/25

Y1 - 2007/10/25

N2 - The present study was carried out to assess the antitumor effect of venom from snake, Hydrophis spiralis on the Ehrlich Ascites Carcinoma (EAC). Four groups of albino Swiss mice were treated with three doses of the sub lethal dose of venom, viz., 0.418, 4.18 and 6.27 μg kg-1 body weight (intraperitoneal injection) along with the standard drug 5 flurouracil (20 mg kg-1 b.w.). The biochemical analysis and rest was left to calculate the mean survival time. In EAC bearing mice, mean life span tumor volume, hemoglobin, red blood cell and lymphocytes were significantly decreased when compared to the normal animals. Whereas, body weight neutrophils and viable tumor cell count was increased in the EAC bearing mice. These changes were brought back to near normal levels in different treatment groups. The macromolecule concentration of peritoneal cells, such as, DNA, RNA and protein, were altered in the EAC bearing mice and observed to be near normal in the treatment groups. The caspase 3 activity was significantly increased in the peritoneal cells of the treatment groups when compared to the EAC bearing mice. The role of apoptotic cascade in EAC cell death was confirmed by the DNA fragmentation on agarose gel. Apart from the antitumor effect, snake venom reduced the tumor burden on the liver and altered the changes in the activities of alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP). Hence the venom from H. Spiralis has a potential antitumor effect on the EAC bearing mice.

AB - The present study was carried out to assess the antitumor effect of venom from snake, Hydrophis spiralis on the Ehrlich Ascites Carcinoma (EAC). Four groups of albino Swiss mice were treated with three doses of the sub lethal dose of venom, viz., 0.418, 4.18 and 6.27 μg kg-1 body weight (intraperitoneal injection) along with the standard drug 5 flurouracil (20 mg kg-1 b.w.). The biochemical analysis and rest was left to calculate the mean survival time. In EAC bearing mice, mean life span tumor volume, hemoglobin, red blood cell and lymphocytes were significantly decreased when compared to the normal animals. Whereas, body weight neutrophils and viable tumor cell count was increased in the EAC bearing mice. These changes were brought back to near normal levels in different treatment groups. The macromolecule concentration of peritoneal cells, such as, DNA, RNA and protein, were altered in the EAC bearing mice and observed to be near normal in the treatment groups. The caspase 3 activity was significantly increased in the peritoneal cells of the treatment groups when compared to the EAC bearing mice. The role of apoptotic cascade in EAC cell death was confirmed by the DNA fragmentation on agarose gel. Apart from the antitumor effect, snake venom reduced the tumor burden on the liver and altered the changes in the activities of alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP). Hence the venom from H. Spiralis has a potential antitumor effect on the EAC bearing mice.

KW - Antitumor

KW - Caspase 3

KW - DNA fragmentation

KW - EAC

KW - Snake venom

UR - http://www.scopus.com/inward/record.url?scp=35349031256&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35349031256&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:35349031256

VL - 3

SP - 167

EP - 173

JO - International Journal of Cancer Research

JF - International Journal of Cancer Research

SN - 1811-9727

IS - 4

ER -