Antrodia salmonea extract inhibits cell proliferation through regulating cell cycle arrest and apoptosis in prostate cancer cell lines

Pang Ting Cheng, Yu Chiao Cheng, Muhammet Oner, Yu Hsuan Li, Mei Chih Chen, Jyh Horng Wu, Ting Chieh Chang, Ayse Celik, Fang Ling Liu, Hsin Yi Wang, Chih Ho Lai, Jer Tsong Hsieh, Chieh Yin Chen, Ho Lin

Research output: Contribution to journalArticlepeer-review

Abstract

Antrodia salmonea (AS) is a fungus, which belongs to a fungal family of Taiwanofungus salmoneus with the features of anti-oxidant, anti-inflammatory, and anticancer. Recent studies have shown that AS has anti-cancer functions in ovarian and breast cancer. However, the effects of AS on prostate cancer (PCa) proliferation remain unknown. Therefore, we investigated the role of AS in PCa proliferation through apoptosis, and cell cycle regulation in PCa cell lines. Our results showed that Antrodia salmonea extract (ASE) inhibited PCa cells growth with a dose-dependent manner. In addition, ASE decreased the anchorage-independent growth formation ability in PC3 cells. Moreover, ASE-induced cell growth inhibition in PCa cells (DU145, PC3) was correlated to decreased cell cycle-related proteins such as cyclin A/B and cyclin-dependent kinase CDK1/2/4, and increased cell cycle inhibitor proteins p21. Besides, ASE decreased the total protein level of epidermal growth factor receptor and its downstream signaling pathways Akt and Erk in both PCa cells. We found that apoptotic markers such as cleaved-PARP protein levels increased significantly in DU145 cells indicating ASE might induce apoptosis. In conclusion, our results suggest that ASE may have the ability to induce PCa cell death through regulating cell cycle arrest and apoptosis pathways.

Original languageEnglish (US)
Pages (from-to)209-214
Number of pages6
JournalChinese Journal of Physiology
Volume65
Issue number4
DOIs
StatePublished - Jul 1 2022

Keywords

  • Anchorage-independent growth
  • Antrodia salmonea
  • apoptosis
  • cell cycle
  • proliferation
  • prostate cancer

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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