AP-1 and clathrin are essential for secretory granule biogenesis in Drosophila

Jason Burgess, Miluska Jauregui, Julie Tan, Janet Rollins, Sylvie Lallet, Peter A. Leventis, Gabrielle L. Boulianne, Henry C. Chang, Roland Le Borgne, Helmut Krämer, Julie A. Brill

Research output: Contribution to journalArticle

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Abstract

Regulated secretion of hormones, digestive enzymes, and other biologically active molecules requires the formation of secretory granules. Clathrin and the clathrin adaptor protein complex 1 (AP-1) are necessary for maturation of exocrine, endocrine, and neuroendocrine secretory granules. However, the initial steps of secretory granule biogenesis are only minimally understood. Powerful genetic approaches available in the fruit fly Drosophila melanogaster were used to investigate the molecular pathway for biogenesis of the mucincontaining "glue granules" that form within epithelial cells of the third-instar larval salivary gland. Clathrin and AP-1 colocalize at the trans-Golgi network (TGN) and clathrin recruitment requires AP-1. Furthermore, clathrin and AP-1 colocalize with secretory cargo at the TGN and on immature granules. Finally, loss of clathrin or AP-1 leads to a profound block in secretory granule formation. These findings establish a novel role for AP-1- and clathrin-dependent trafficking in the biogenesis of mucin-containing secretory granules.

Original languageEnglish (US)
Pages (from-to)2094-2105
Number of pages12
JournalMolecular Biology of the Cell
Volume22
Issue number12
DOIs
StatePublished - Jun 15 2011

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Adaptor Protein Complex 1
Secretory Vesicles
Drosophila
trans-Golgi Network
Clathrin
Mucins
Salivary Glands
Drosophila melanogaster
Diptera
Adhesives
Fruit
Epithelial Cells
Hormones

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Burgess, J., Jauregui, M., Tan, J., Rollins, J., Lallet, S., Leventis, P. A., ... Brill, J. A. (2011). AP-1 and clathrin are essential for secretory granule biogenesis in Drosophila. Molecular Biology of the Cell, 22(12), 2094-2105. https://doi.org/10.1091/mbc.E11-01-0054

AP-1 and clathrin are essential for secretory granule biogenesis in Drosophila. / Burgess, Jason; Jauregui, Miluska; Tan, Julie; Rollins, Janet; Lallet, Sylvie; Leventis, Peter A.; Boulianne, Gabrielle L.; Chang, Henry C.; Borgne, Roland Le; Krämer, Helmut; Brill, Julie A.

In: Molecular Biology of the Cell, Vol. 22, No. 12, 15.06.2011, p. 2094-2105.

Research output: Contribution to journalArticle

Burgess, J, Jauregui, M, Tan, J, Rollins, J, Lallet, S, Leventis, PA, Boulianne, GL, Chang, HC, Borgne, RL, Krämer, H & Brill, JA 2011, 'AP-1 and clathrin are essential for secretory granule biogenesis in Drosophila', Molecular Biology of the Cell, vol. 22, no. 12, pp. 2094-2105. https://doi.org/10.1091/mbc.E11-01-0054
Burgess J, Jauregui M, Tan J, Rollins J, Lallet S, Leventis PA et al. AP-1 and clathrin are essential for secretory granule biogenesis in Drosophila. Molecular Biology of the Cell. 2011 Jun 15;22(12):2094-2105. https://doi.org/10.1091/mbc.E11-01-0054
Burgess, Jason ; Jauregui, Miluska ; Tan, Julie ; Rollins, Janet ; Lallet, Sylvie ; Leventis, Peter A. ; Boulianne, Gabrielle L. ; Chang, Henry C. ; Borgne, Roland Le ; Krämer, Helmut ; Brill, Julie A. / AP-1 and clathrin are essential for secretory granule biogenesis in Drosophila. In: Molecular Biology of the Cell. 2011 ; Vol. 22, No. 12. pp. 2094-2105.
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