APC-resistance as measured by a Textarin time assay: Comparison to the APTT-based method

L. E. Hoagland, Douglas A. Triplett, F. Peng, L. Barna

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Protein C is a major regulatory protein critical to physiologic anticoagulation. When activated, it selectively degrades the activated forms of factors V and VIII, thereby, down-regulating blood coagulation. Using an activated partial thromboplastin time (ATPP) assay, Dahlbeck et al. recently reported that some individuals with thrombophilia show a poor in vitro anticoagulant response to activated protein C (APC-Resistance). Subsequent studies identified a point mutation in the gene for factor V as the underlying cause of APC-Resistance. The incidence of APC-Resistance in patients with recurrent thromboembolic events approached 50%. The APC-Resistance phenotype is also present in approximately 5% of normal Caucasian subjects. In an attempt to develop a more sensitive and specific test system, we evaluated an assay based on Textarin®(Pentapharm, Basel, Switzerland). Textarin®, a protein fraction of Pseudonaja textilis venom (Australian Eastern Brown Snake) activates prothrombin in the presence of phospholipid (PL), factor V and calcium ion. Based on Textarin's requirement for factor V, we developed a Textarin time assay to test for APC-Resistance. We evaluated this test system in normal subjects and the following patient populations: stable orally anticoagulated, previously diagnosed factor V Leiden, and therapeutically heparinized samples. We found the Textarin assay to be a sensitive and specific test system to identify APC-Resistance. The phenotypic Textarin APC-Resistance test correlated more closely with the genotypic abnormality of factor V(506Q) than the APTT-APC-Resistance test.

Original languageEnglish (US)
Pages (from-to)363-373
Number of pages11
JournalThrombosis Research
Volume83
Issue number5
DOIs
StatePublished - Sep 1 1996

Fingerprint

Activated Protein C Resistance
Factor V
Factor VIIIa
Factor Va
textilotoxin
Thrombophilia
Partial Thromboplastin Time
Snakes
Venoms
Population Dynamics
Prothrombin
Blood Coagulation
Protein C
Switzerland
Point Mutation
Anticoagulants
Phospholipids
Proteins
Ions
Calcium

Keywords

  • Activated protein C resistance
  • Factor V mutation
  • Textarin®

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

Cite this

APC-resistance as measured by a Textarin time assay : Comparison to the APTT-based method. / Hoagland, L. E.; Triplett, Douglas A.; Peng, F.; Barna, L.

In: Thrombosis Research, Vol. 83, No. 5, 01.09.1996, p. 363-373.

Research output: Contribution to journalArticle

Hoagland, L. E. ; Triplett, Douglas A. ; Peng, F. ; Barna, L. / APC-resistance as measured by a Textarin time assay : Comparison to the APTT-based method. In: Thrombosis Research. 1996 ; Vol. 83, No. 5. pp. 363-373.
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