Apical constriction drives tissue-scale hydrodynamic flow to mediate cell elongation

Bing He, Konstantin Doubrovinski, Oleg Polyakov, Eric Wieschaus

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Epithelial folding mediated by apical constriction converts flat epithelial sheets into multilayered, complex tissue structures and is used throughout development in most animals. Little is known, however, about how forces produced near the apical surface of the tissue are transmitted within individual cells to generate the global changes in cell shape that characterize tissue deformation. Here we apply particle tracking velocimetry in gastrulating Drosophila embryos to measure the movement of cytoplasm and plasma membrane during ventral furrow formation. We find that cytoplasmic redistribution during the lengthening phase of ventral furrow formation can be precisely described by viscous flows that quantitatively match the predictions of hydrodynamics. Cell membranes move with the ambient cytoplasm, with little resistance to, or driving force on, the flow. Strikingly, apical constriction produces similar flow patterns in mutant embryos that fail to form cells before gastrulation (acellular'embryos), such that the global redistribution of cytoplasm mirrors the summed redistribution occurring in individual cells of wild-type embryos. Our results indicate that during the lengthening phase of ventral furrow formation, hydrodynamic behaviour of the cytoplasm provides the predominant mechanism transmitting apically generated forces deep into the tissue and that cell individualization is dispensable.

Original languageEnglish (US)
Pages (from-to)392-396
Number of pages5
JournalNature
Volume508
Issue number7496
DOIs
StatePublished - Jan 1 2014

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Hydrodynamics
Constriction
Cytoplasm
Embryonic Structures
Cell Membrane
Gastrulation
Cell Shape
Rheology
Drosophila
Drive

ASJC Scopus subject areas

  • General

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Apical constriction drives tissue-scale hydrodynamic flow to mediate cell elongation. / He, Bing; Doubrovinski, Konstantin; Polyakov, Oleg; Wieschaus, Eric.

In: Nature, Vol. 508, No. 7496, 01.01.2014, p. 392-396.

Research output: Contribution to journalArticle

He, Bing ; Doubrovinski, Konstantin ; Polyakov, Oleg ; Wieschaus, Eric. / Apical constriction drives tissue-scale hydrodynamic flow to mediate cell elongation. In: Nature. 2014 ; Vol. 508, No. 7496. pp. 392-396.
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