Apoptosis: a possible mechanism of suppressing parathyroid hyperplasia by calcimimetics

Masahide Mizobuchi, Fumie Saji, Kazuhiro Shiizaki, Toshihiro Sato, Maki Shibata, Shigeo Negi, Tadao Akizawa, Ikuji Hatamura, Hiroaki Ogata, Eriko Kinugasa

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2 Scopus citations

Abstract

The mechanism through which calcimimetic compounds suppress parathyroid cell growth has not been fully elucidated. We investigated the effect of the calcimimetic compound (NPS R-568:R568) on the parathyroid cell growth in vitro. Whole parathyroid glands of subtotally nephrectomized rats fed high phosphorus diet for 8 weeks were used in this study. Fresh rat parathyroid glands were incubated in a media (phosphorus concentration is 1.0 mM and calcium is 1.25 mM) with R-568 (10-4 M) or calcitriol (10-7 M) or vehicle for 6 hours. Medium PTH level was significantly decreased in both R568-treated group and calcitriol-treated group compared with either vehicle-treated group, or no-treated group. While TUNEL-positive cells were similar in calcitriol-treated group compared with both vehicle-treated and no-treated groups, they were significantly increased in R568-treated group compared with other three groups. These results suggest that calcimimetic compounds, NPS R-568 reduces PTH secretion and induces apoptosis of hyperfunctional parathyroid cell in vitro.

Original languageEnglish (US)
Pages (from-to)56-58; discussion 58-59
JournalClinical calcium
Volume15 Suppl 1
StatePublished - Sep 2005

ASJC Scopus subject areas

  • Medicine(all)

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    Mizobuchi, M., Saji, F., Shiizaki, K., Sato, T., Shibata, M., Negi, S., Akizawa, T., Hatamura, I., Ogata, H., & Kinugasa, E. (2005). Apoptosis: a possible mechanism of suppressing parathyroid hyperplasia by calcimimetics. Clinical calcium, 15 Suppl 1, 56-58; discussion 58-59.