Apoptosis: a possible mechanism of suppressing parathyroid hyperplasia by calcimimetics

Masahide Mizobuchi, Fumie Saji, Kazuhiro Shiizaki, Toshihiro Sato, Maki Shibata, Shigeo Negi, Tadao Akizawa, Ikuji Hatamura, Hiroaki Ogata, Eriko Kinugasa

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2 Citations (Scopus)

Abstract

The mechanism through which calcimimetic compounds suppress parathyroid cell growth has not been fully elucidated. We investigated the effect of the calcimimetic compound (NPS R-568:R568) on the parathyroid cell growth in vitro. Whole parathyroid glands of subtotally nephrectomized rats fed high phosphorus diet for 8 weeks were used in this study. Fresh rat parathyroid glands were incubated in a media (phosphorus concentration is 1.0 mM and calcium is 1.25 mM) with R-568 (10-4 M) or calcitriol (10-7 M) or vehicle for 6 hours. Medium PTH level was significantly decreased in both R568-treated group and calcitriol-treated group compared with either vehicle-treated group, or no-treated group. While TUNEL-positive cells were similar in calcitriol-treated group compared with both vehicle-treated and no-treated groups, they were significantly increased in R568-treated group compared with other three groups. These results suggest that calcimimetic compounds, NPS R-568 reduces PTH secretion and induces apoptosis of hyperfunctional parathyroid cell in vitro.

Original languageEnglish (US)
JournalClinical calcium
Volume15 Suppl 1
StatePublished - 2005

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N-(2-chlorophenylpropyl)-1-(3-methoxyphenyl)ethylamine
Hyperplasia
Apoptosis
Calcitriol
Parathyroid Glands
Phosphorus
In Situ Nick-End Labeling
Growth
Diet
Calcium

Cite this

Mizobuchi, M., Saji, F., Shiizaki, K., Sato, T., Shibata, M., Negi, S., ... Kinugasa, E. (2005). Apoptosis: a possible mechanism of suppressing parathyroid hyperplasia by calcimimetics. Clinical calcium, 15 Suppl 1.

Apoptosis : a possible mechanism of suppressing parathyroid hyperplasia by calcimimetics. / Mizobuchi, Masahide; Saji, Fumie; Shiizaki, Kazuhiro; Sato, Toshihiro; Shibata, Maki; Negi, Shigeo; Akizawa, Tadao; Hatamura, Ikuji; Ogata, Hiroaki; Kinugasa, Eriko.

In: Clinical calcium, Vol. 15 Suppl 1, 2005.

Research output: Contribution to journalArticle

Mizobuchi, M, Saji, F, Shiizaki, K, Sato, T, Shibata, M, Negi, S, Akizawa, T, Hatamura, I, Ogata, H & Kinugasa, E 2005, 'Apoptosis: a possible mechanism of suppressing parathyroid hyperplasia by calcimimetics', Clinical calcium, vol. 15 Suppl 1.
Mizobuchi M, Saji F, Shiizaki K, Sato T, Shibata M, Negi S et al. Apoptosis: a possible mechanism of suppressing parathyroid hyperplasia by calcimimetics. Clinical calcium. 2005;15 Suppl 1.
Mizobuchi, Masahide ; Saji, Fumie ; Shiizaki, Kazuhiro ; Sato, Toshihiro ; Shibata, Maki ; Negi, Shigeo ; Akizawa, Tadao ; Hatamura, Ikuji ; Ogata, Hiroaki ; Kinugasa, Eriko. / Apoptosis : a possible mechanism of suppressing parathyroid hyperplasia by calcimimetics. In: Clinical calcium. 2005 ; Vol. 15 Suppl 1.
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