Application of high-performance liquid chromatography for the study of the microheterogeneity changes of mouse α-fetoprotein in fetal development

L. T. Wong; C. Y. Lu; D. O. Tinker; J. C. Hsia

doi:10.1016/0165-022X(88)90014-0

Application of high-performance liquid chromatography for the study of the microheterogeneity changes of mouse α-fetoprotein in fetal development

L. T. Wong, C. Y. Lu, D. O. Tinker, J. C. Hsia

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Changes in the microheterogeneity of mouse α-fetoprotein (MAFP) during fetal development were investigated by high-performance liquid chromatography (HPLC). A total of six distinct isoforms (Iso-1, Iso-2, Iso-3, Iso-4, Iso-5, Iso-6) of the heterogeneous MAFP were resolved from mouse amniotic fluid (MAF). Analysis of MAF collected at various times revealed that these isoforms were accumulated at different stages of the fetal development.

Original language	English (US)
Pages (from-to)	267-272
Number of pages	6
Journal	Journal of Biochemical and Biophysical Methods
Volume	15
Issue number	5
DOIs	https://doi.org/10.1016/0165-022X(88)90014-0
State	Published - Mar 1988

Keywords

Anion-exchange column
Fetal development
High-performance liquid chromatography
Isoform
Microheterogeneity
α-Fetoprotein

ASJC Scopus subject areas

Biophysics
Biochemistry

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10.1016/0165-022X(88)90014-0

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@article{f0aff4f024504548a0b46c50e2328d02,

title = "Application of high-performance liquid chromatography for the study of the microheterogeneity changes of mouse α-fetoprotein in fetal development",

abstract = "Changes in the microheterogeneity of mouse α-fetoprotein (MAFP) during fetal development were investigated by high-performance liquid chromatography (HPLC). A total of six distinct isoforms (Iso-1, Iso-2, Iso-3, Iso-4, Iso-5, Iso-6) of the heterogeneous MAFP were resolved from mouse amniotic fluid (MAF). Analysis of MAF collected at various times revealed that these isoforms were accumulated at different stages of the fetal development.",

keywords = "Anion-exchange column, Fetal development, High-performance liquid chromatography, Isoform, Microheterogeneity, α-Fetoprotein",

author = "Wong, {L. T.} and Lu, {C. Y.} and Tinker, {D. O.} and Hsia, {J. C.}",

note = "Funding Information: This research is supported in part by a grant (No. MA-4129) from the Medical Research Council of Canada. We thank Mr. Li Qian for his excellent technical assistance. This research is also partly supported by National Institute of Health Grant No. HD20188 to C.Y.L. who is a Fellow of the Hartford Foundation.",

year = "1988",

month = mar,

doi = "10.1016/0165-022X(88)90014-0",

language = "English (US)",

volume = "15",

pages = "267--272",

journal = "Journal of Biochemical and Biophysical Methods",

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T1 - Application of high-performance liquid chromatography for the study of the microheterogeneity changes of mouse α-fetoprotein in fetal development

AU - Wong, L. T.

AU - Lu, C. Y.

AU - Tinker, D. O.

AU - Hsia, J. C.

N1 - Funding Information: This research is supported in part by a grant (No. MA-4129) from the Medical Research Council of Canada. We thank Mr. Li Qian for his excellent technical assistance. This research is also partly supported by National Institute of Health Grant No. HD20188 to C.Y.L. who is a Fellow of the Hartford Foundation.

PY - 1988/3

Y1 - 1988/3

N2 - Changes in the microheterogeneity of mouse α-fetoprotein (MAFP) during fetal development were investigated by high-performance liquid chromatography (HPLC). A total of six distinct isoforms (Iso-1, Iso-2, Iso-3, Iso-4, Iso-5, Iso-6) of the heterogeneous MAFP were resolved from mouse amniotic fluid (MAF). Analysis of MAF collected at various times revealed that these isoforms were accumulated at different stages of the fetal development.

AB - Changes in the microheterogeneity of mouse α-fetoprotein (MAFP) during fetal development were investigated by high-performance liquid chromatography (HPLC). A total of six distinct isoforms (Iso-1, Iso-2, Iso-3, Iso-4, Iso-5, Iso-6) of the heterogeneous MAFP were resolved from mouse amniotic fluid (MAF). Analysis of MAF collected at various times revealed that these isoforms were accumulated at different stages of the fetal development.

KW - Anion-exchange column

KW - Fetal development

KW - High-performance liquid chromatography

KW - Isoform

KW - Microheterogeneity

KW - α-Fetoprotein

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ER -

Application of high-performance liquid chromatography for the study of the microheterogeneity changes of mouse α-fetoprotein in fetal development

Abstract

Keywords

ASJC Scopus subject areas

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