Approach to the assessment of platelet function: Comparison between optical-based platelet-rich plasma and impedance-based whole blood platelet aggregation methods

Anna M. Dyszkiewicz-Korpanty, Eugene P. Frenkel, Ravindra Sarode

Research output: Contribution to journalArticle

91 Scopus citations


Platelet aggregation studies play an important role in the assessment of hereditary and acquired platelet function defects. The first aggregation test introduced into laboratory practice used platelet-rich plasma (PRP) where aggregation was detected by an optical method. The assessment of platelet function using whole blood (WB) aggregation by an impedance method followed up nearly 20 years later. The WB impedance aggregation assay appears to be superior to the optical method because it 1) evaluates platelets in a physiologic milieu in the presence of red and white blood cells, which are known to modulate platelet function; 2) is faster; 3) has higher sensitivity; and 4) does not require centrifugation, thus avoiding injury to platelets and loss of giant thrombocytes. These two assays were compared. Clearly, the WB impedance aggregation methodology has many advantages over the optical PRP assay for the assessment of the hyperactive platelet syndrome and the effects of antiplatelet drugs.

Original languageEnglish (US)
Pages (from-to)25-35
Number of pages11
JournalClinical and Applied Thrombosis/Hemostasis
Issue number1
Publication statusPublished - Jan 2005



  • Antiplatelet drugs
  • Hyperactive platelet syndrome
  • Optical platelet aggregation
  • Whole blood impedance aggregation

ASJC Scopus subject areas

  • Hematology

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