Aptasensor for Quantifying Pancreatic Polypeptide

Ahmed S.M. Ali, Medhat S. El-Halawany, Sherif Abdelaziz Ibrahim, Olga Plückthun, Ahmed S.G. Khalil, Günter Mayer

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Herein, a simple colorimetric aptasensor based on unmodified gold nanoparticles (AuNPs) has been developed for quantifying pancreatic polypeptide (PP), a peptide hormone associated with nonfunctional neuroendocrine pancreatic tumor (NF-PNET). First, several PP-specific aptamers have been screened by systematic evolution of ligands by exponential enrichment. The generated aptamers showed specificity and affinity higher than those reported so far. The sensing platform was constructed based on the optical properties of AuNPs as well as the adsorption of single-stranded DNA aptamers. The high sensitivity of the developed aptasensor was achieved by optimizing the sensing conditions, including salt tolerance, time of reaction, and optimal aptamer concentration. The developed aptasensor showed a wide linear dynamic range (1-60 nM) with a limit of detection of 521 pM. Furthermore, the performance of the aptasensor was evaluated for the detection of PP in human serum and showed recovery values ranging between 92.4 and 93.9%. Therefore, the presented colorimetric aptasensor has a great potential in the diagnosis of NF-pNETs.

Original languageEnglish (US)
Pages (from-to)2948-2956
Number of pages9
JournalACS Omega
Volume4
Issue number2
DOIs
StatePublished - Feb 8 2019
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Fingerprint Dive into the research topics of 'Aptasensor for Quantifying Pancreatic Polypeptide'. Together they form a unique fingerprint.

  • Cite this

    Ali, A. S. M., El-Halawany, M. S., Ibrahim, S. A., Plückthun, O., Khalil, A. S. G., & Mayer, G. (2019). Aptasensor for Quantifying Pancreatic Polypeptide. ACS Omega, 4(2), 2948-2956. https://doi.org/10.1021/acsomega.8b03131