Aqueous humor sCD44 concentration and visual field loss in primary open-angle glaucoma

Michael J. Nolan, Michael C. Giovingo, Adam M. Miller, Robert D. Wertz, Robert Ritch, Jeffrey M. Liebmann, R. Rand Allingham, Leon W. Herndon, Martin B. Wax, Regina Smolyak, Fareed Hasan, Edward M. Barnett, John R. Samples, Paul A. Knepper

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

PURPOSE: To correlate aqueous humor soluble CD44 (sCD44) concentration, visual field loss, and glaucoma risk factors in primary open-angle glaucoma (POAG) patients. METHODS: Aqueous samples were obtained by paracentesis from normal and glaucoma patients who were undergoing elective surgery and analyzed for sCD44 concentration by enzyme-linked immunosorbent assay. RESULTS: In normal aqueous (n=124) the sCD44 concentration was 5.88±0.27 ng/mL, whereas in POAG aqueous (n=90) the sCD44 concentration was 12.76±0.66 ng/mL, a 2.2-fold increase (P<0.000001). In POAG patients with prior successful filtration surgery (n=13), the sCD44 concentration was decreased by 43% to 7.32±1.44 (P=0.001) in comparison with POAG patients without filtration surgery; however, the sCD44 concentration in the prior successful filtration subgroup with no medications and normal intraocular pressure was 12.62±3.81 (P=0.05) compared with normal. The sCD44 concentration of normal pressure glaucoma patients was 9.19±1.75 ng/mL, a 1.6-fold increase compared with normal (P=0.02). Race and intraocular pressure pulse amplitude were significant POAG risk factors in this cohort of patients. In both normal and POAG patients with mild and moderate visual field loss, sCD44 concentration was greater in African Americans than in whites (P=0.04) CONCLUSIONS: sCD44 concentration in the aqueous of POAG patients correlated with the severity of visual field loss in all stages in white patients and in mild to moderate stages in African American patients. sCD44 concentration in aqueous is a possible protein biomarker of visual field loss in POAG.

Original languageEnglish (US)
Pages (from-to)419-429
Number of pages11
JournalJournal of Glaucoma
Volume16
Issue number5
DOIs
StatePublished - Aug 2007

Fingerprint

Aqueous Humor
Visual Fields
Glaucoma
Filtering Surgery
Intraocular Pressure
African Americans
Primary Open Angle Glaucoma
Paracentesis
Biomarkers
Enzyme-Linked Immunosorbent Assay
Pressure

Keywords

  • CD44
  • Glaucoma
  • Risk factors
  • Visual fields

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Nolan, M. J., Giovingo, M. C., Miller, A. M., Wertz, R. D., Ritch, R., Liebmann, J. M., ... Knepper, P. A. (2007). Aqueous humor sCD44 concentration and visual field loss in primary open-angle glaucoma. Journal of Glaucoma, 16(5), 419-429. https://doi.org/10.1097/IJG.0b013e318050ab4b

Aqueous humor sCD44 concentration and visual field loss in primary open-angle glaucoma. / Nolan, Michael J.; Giovingo, Michael C.; Miller, Adam M.; Wertz, Robert D.; Ritch, Robert; Liebmann, Jeffrey M.; Rand Allingham, R.; Herndon, Leon W.; Wax, Martin B.; Smolyak, Regina; Hasan, Fareed; Barnett, Edward M.; Samples, John R.; Knepper, Paul A.

In: Journal of Glaucoma, Vol. 16, No. 5, 08.2007, p. 419-429.

Research output: Contribution to journalArticle

Nolan, MJ, Giovingo, MC, Miller, AM, Wertz, RD, Ritch, R, Liebmann, JM, Rand Allingham, R, Herndon, LW, Wax, MB, Smolyak, R, Hasan, F, Barnett, EM, Samples, JR & Knepper, PA 2007, 'Aqueous humor sCD44 concentration and visual field loss in primary open-angle glaucoma', Journal of Glaucoma, vol. 16, no. 5, pp. 419-429. https://doi.org/10.1097/IJG.0b013e318050ab4b
Nolan MJ, Giovingo MC, Miller AM, Wertz RD, Ritch R, Liebmann JM et al. Aqueous humor sCD44 concentration and visual field loss in primary open-angle glaucoma. Journal of Glaucoma. 2007 Aug;16(5):419-429. https://doi.org/10.1097/IJG.0b013e318050ab4b
Nolan, Michael J. ; Giovingo, Michael C. ; Miller, Adam M. ; Wertz, Robert D. ; Ritch, Robert ; Liebmann, Jeffrey M. ; Rand Allingham, R. ; Herndon, Leon W. ; Wax, Martin B. ; Smolyak, Regina ; Hasan, Fareed ; Barnett, Edward M. ; Samples, John R. ; Knepper, Paul A. / Aqueous humor sCD44 concentration and visual field loss in primary open-angle glaucoma. In: Journal of Glaucoma. 2007 ; Vol. 16, No. 5. pp. 419-429.
@article{fb554de454cd4dd1b73558d02a5eeb96,
title = "Aqueous humor sCD44 concentration and visual field loss in primary open-angle glaucoma",
abstract = "PURPOSE: To correlate aqueous humor soluble CD44 (sCD44) concentration, visual field loss, and glaucoma risk factors in primary open-angle glaucoma (POAG) patients. METHODS: Aqueous samples were obtained by paracentesis from normal and glaucoma patients who were undergoing elective surgery and analyzed for sCD44 concentration by enzyme-linked immunosorbent assay. RESULTS: In normal aqueous (n=124) the sCD44 concentration was 5.88±0.27 ng/mL, whereas in POAG aqueous (n=90) the sCD44 concentration was 12.76±0.66 ng/mL, a 2.2-fold increase (P<0.000001). In POAG patients with prior successful filtration surgery (n=13), the sCD44 concentration was decreased by 43{\%} to 7.32±1.44 (P=0.001) in comparison with POAG patients without filtration surgery; however, the sCD44 concentration in the prior successful filtration subgroup with no medications and normal intraocular pressure was 12.62±3.81 (P=0.05) compared with normal. The sCD44 concentration of normal pressure glaucoma patients was 9.19±1.75 ng/mL, a 1.6-fold increase compared with normal (P=0.02). Race and intraocular pressure pulse amplitude were significant POAG risk factors in this cohort of patients. In both normal and POAG patients with mild and moderate visual field loss, sCD44 concentration was greater in African Americans than in whites (P=0.04) CONCLUSIONS: sCD44 concentration in the aqueous of POAG patients correlated with the severity of visual field loss in all stages in white patients and in mild to moderate stages in African American patients. sCD44 concentration in aqueous is a possible protein biomarker of visual field loss in POAG.",
keywords = "CD44, Glaucoma, Risk factors, Visual fields",
author = "Nolan, {Michael J.} and Giovingo, {Michael C.} and Miller, {Adam M.} and Wertz, {Robert D.} and Robert Ritch and Liebmann, {Jeffrey M.} and {Rand Allingham}, R. and Herndon, {Leon W.} and Wax, {Martin B.} and Regina Smolyak and Fareed Hasan and Barnett, {Edward M.} and Samples, {John R.} and Knepper, {Paul A.}",
year = "2007",
month = "8",
doi = "10.1097/IJG.0b013e318050ab4b",
language = "English (US)",
volume = "16",
pages = "419--429",
journal = "Journal of Glaucoma",
issn = "1057-0829",
publisher = "Lippincott Williams and Wilkins",
number = "5",

}

TY - JOUR

T1 - Aqueous humor sCD44 concentration and visual field loss in primary open-angle glaucoma

AU - Nolan, Michael J.

AU - Giovingo, Michael C.

AU - Miller, Adam M.

AU - Wertz, Robert D.

AU - Ritch, Robert

AU - Liebmann, Jeffrey M.

AU - Rand Allingham, R.

AU - Herndon, Leon W.

AU - Wax, Martin B.

AU - Smolyak, Regina

AU - Hasan, Fareed

AU - Barnett, Edward M.

AU - Samples, John R.

AU - Knepper, Paul A.

PY - 2007/8

Y1 - 2007/8

N2 - PURPOSE: To correlate aqueous humor soluble CD44 (sCD44) concentration, visual field loss, and glaucoma risk factors in primary open-angle glaucoma (POAG) patients. METHODS: Aqueous samples were obtained by paracentesis from normal and glaucoma patients who were undergoing elective surgery and analyzed for sCD44 concentration by enzyme-linked immunosorbent assay. RESULTS: In normal aqueous (n=124) the sCD44 concentration was 5.88±0.27 ng/mL, whereas in POAG aqueous (n=90) the sCD44 concentration was 12.76±0.66 ng/mL, a 2.2-fold increase (P<0.000001). In POAG patients with prior successful filtration surgery (n=13), the sCD44 concentration was decreased by 43% to 7.32±1.44 (P=0.001) in comparison with POAG patients without filtration surgery; however, the sCD44 concentration in the prior successful filtration subgroup with no medications and normal intraocular pressure was 12.62±3.81 (P=0.05) compared with normal. The sCD44 concentration of normal pressure glaucoma patients was 9.19±1.75 ng/mL, a 1.6-fold increase compared with normal (P=0.02). Race and intraocular pressure pulse amplitude were significant POAG risk factors in this cohort of patients. In both normal and POAG patients with mild and moderate visual field loss, sCD44 concentration was greater in African Americans than in whites (P=0.04) CONCLUSIONS: sCD44 concentration in the aqueous of POAG patients correlated with the severity of visual field loss in all stages in white patients and in mild to moderate stages in African American patients. sCD44 concentration in aqueous is a possible protein biomarker of visual field loss in POAG.

AB - PURPOSE: To correlate aqueous humor soluble CD44 (sCD44) concentration, visual field loss, and glaucoma risk factors in primary open-angle glaucoma (POAG) patients. METHODS: Aqueous samples were obtained by paracentesis from normal and glaucoma patients who were undergoing elective surgery and analyzed for sCD44 concentration by enzyme-linked immunosorbent assay. RESULTS: In normal aqueous (n=124) the sCD44 concentration was 5.88±0.27 ng/mL, whereas in POAG aqueous (n=90) the sCD44 concentration was 12.76±0.66 ng/mL, a 2.2-fold increase (P<0.000001). In POAG patients with prior successful filtration surgery (n=13), the sCD44 concentration was decreased by 43% to 7.32±1.44 (P=0.001) in comparison with POAG patients without filtration surgery; however, the sCD44 concentration in the prior successful filtration subgroup with no medications and normal intraocular pressure was 12.62±3.81 (P=0.05) compared with normal. The sCD44 concentration of normal pressure glaucoma patients was 9.19±1.75 ng/mL, a 1.6-fold increase compared with normal (P=0.02). Race and intraocular pressure pulse amplitude were significant POAG risk factors in this cohort of patients. In both normal and POAG patients with mild and moderate visual field loss, sCD44 concentration was greater in African Americans than in whites (P=0.04) CONCLUSIONS: sCD44 concentration in the aqueous of POAG patients correlated with the severity of visual field loss in all stages in white patients and in mild to moderate stages in African American patients. sCD44 concentration in aqueous is a possible protein biomarker of visual field loss in POAG.

KW - CD44

KW - Glaucoma

KW - Risk factors

KW - Visual fields

UR - http://www.scopus.com/inward/record.url?scp=34547912469&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547912469&partnerID=8YFLogxK

U2 - 10.1097/IJG.0b013e318050ab4b

DO - 10.1097/IJG.0b013e318050ab4b

M3 - Article

C2 - 17700283

AN - SCOPUS:34547912469

VL - 16

SP - 419

EP - 429

JO - Journal of Glaucoma

JF - Journal of Glaucoma

SN - 1057-0829

IS - 5

ER -