Arachidonic acid epoxygenase: detection of epoxyeicosatrienoic acids in human urine

Robert Toto; Arup Siddhanta; Sukumar Manna; Bikash Pramanik; J. R. Faick; Jorge Capdevila

doi:10.1016/0005-2760(87)90199-8

Arachidonic acid epoxygenase: detection of epoxyeicosatrienoic acids in human urine

Robert Toto, Arup Siddhanta, Sukumar Manna, Bikash Pramanik, J. R. Faick, Jorge Capdevila

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Epoxyeicosatrienoic acids, metabolites of the cytochrome P-450-mediated epoxygenase reaction, were detected in human urine by gas chromatographic-mass spectroscopic techniques after conversion to their hydrogenated and non-hydrogenated methyl and pentafluorobenzyl esters. Initial analysis of the regioisomeric composition utilizing the corresponding hydrogenated pentafluorobenzyl esters revealed the presence of the 8,9- and 14,15-isomers.

Original language	English (US)
Pages (from-to)	132-139
Number of pages	8
Journal	Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume	919
Issue number	2
DOIs	https://doi.org/10.1016/0005-2760(87)90199-8
State	Published - Jun 2 1987

Keywords

(Human)
Arachidonic acid
Epoxygenase
Epoxyicosatrienoic acid
Urinary metabolite

ASJC Scopus subject areas

Biophysics
Biochemistry
Endocrinology

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10.1016/0005-2760(87)90199-8

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title = "Arachidonic acid epoxygenase: detection of epoxyeicosatrienoic acids in human urine",

abstract = "Epoxyeicosatrienoic acids, metabolites of the cytochrome P-450-mediated epoxygenase reaction, were detected in human urine by gas chromatographic-mass spectroscopic techniques after conversion to their hydrogenated and non-hydrogenated methyl and pentafluorobenzyl esters. Initial analysis of the regioisomeric composition utilizing the corresponding hydrogenated pentafluorobenzyl esters revealed the presence of the 8,9- and 14,15-isomers.",

keywords = "(Human), Arachidonic acid, Epoxygenase, Epoxyicosatrienoic acid, Urinary metabolite",

author = "Robert Toto and Arup Siddhanta and Sukumar Manna and Bikash Pramanik and Faick, {J. R.} and Jorge Capdevila",

note = "Funding Information: Supported by grants USPHS NIADDKD- 38226, R23 AM36319 OlAl, and the Robert A. Welch Foundation (I-782).",

year = "1987",

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doi = "10.1016/0005-2760(87)90199-8",

language = "English (US)",

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TY - JOUR

T1 - Arachidonic acid epoxygenase

T2 - detection of epoxyeicosatrienoic acids in human urine

AU - Toto, Robert

AU - Siddhanta, Arup

AU - Manna, Sukumar

AU - Pramanik, Bikash

AU - Faick, J. R.

AU - Capdevila, Jorge

N1 - Funding Information: Supported by grants USPHS NIADDKD- 38226, R23 AM36319 OlAl, and the Robert A. Welch Foundation (I-782).

PY - 1987/6/2

Y1 - 1987/6/2

N2 - Epoxyeicosatrienoic acids, metabolites of the cytochrome P-450-mediated epoxygenase reaction, were detected in human urine by gas chromatographic-mass spectroscopic techniques after conversion to their hydrogenated and non-hydrogenated methyl and pentafluorobenzyl esters. Initial analysis of the regioisomeric composition utilizing the corresponding hydrogenated pentafluorobenzyl esters revealed the presence of the 8,9- and 14,15-isomers.

AB - Epoxyeicosatrienoic acids, metabolites of the cytochrome P-450-mediated epoxygenase reaction, were detected in human urine by gas chromatographic-mass spectroscopic techniques after conversion to their hydrogenated and non-hydrogenated methyl and pentafluorobenzyl esters. Initial analysis of the regioisomeric composition utilizing the corresponding hydrogenated pentafluorobenzyl esters revealed the presence of the 8,9- and 14,15-isomers.

KW - (Human)

KW - Arachidonic acid

KW - Epoxygenase

KW - Epoxyicosatrienoic acid

KW - Urinary metabolite

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Arachidonic acid epoxygenase: detection of epoxyeicosatrienoic acids in human urine

Abstract

Keywords

ASJC Scopus subject areas

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