Arachidonic acid epoxygenase: detection of epoxyeicosatrienoic acids in human urine

Robert Toto, Arup Siddhanta, Sukumar Manna, Bikash Pramanik, J. R. Faick, Jorge Capdevila

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Epoxyeicosatrienoic acids, metabolites of the cytochrome P-450-mediated epoxygenase reaction, were detected in human urine by gas chromatographic-mass spectroscopic techniques after conversion to their hydrogenated and non-hydrogenated methyl and pentafluorobenzyl esters. Initial analysis of the regioisomeric composition utilizing the corresponding hydrogenated pentafluorobenzyl esters revealed the presence of the 8,9- and 14,15-isomers.

Original languageEnglish (US)
Pages (from-to)132-139
Number of pages8
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume919
Issue number2
DOIs
StatePublished - Jun 2 1987

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Esters
Urine
Acids
Metabolites
Isomers
Cytochrome P-450 Enzyme System
Gases
Chemical analysis
arachidonate epoxygenase

Keywords

  • (Human)
  • Arachidonic acid
  • Epoxygenase
  • Epoxyicosatrienoic acid
  • Urinary metabolite

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Endocrinology

Cite this

Arachidonic acid epoxygenase : detection of epoxyeicosatrienoic acids in human urine. / Toto, Robert; Siddhanta, Arup; Manna, Sukumar; Pramanik, Bikash; Faick, J. R.; Capdevila, Jorge.

In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, Vol. 919, No. 2, 02.06.1987, p. 132-139.

Research output: Contribution to journalArticle

Toto, Robert ; Siddhanta, Arup ; Manna, Sukumar ; Pramanik, Bikash ; Faick, J. R. ; Capdevila, Jorge. / Arachidonic acid epoxygenase : detection of epoxyeicosatrienoic acids in human urine. In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism. 1987 ; Vol. 919, No. 2. pp. 132-139.
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