Arachidonic acid epoxygenase. Stereochemical analysis of the endogenous epoxyeicosatrienoic acids of human kidney cortex

Armando Karara; Elizabeth Dishman; Harry Jacobson; J. R. Falck; Jorge H. Capdevila

doi:10.1016/0014-5793(90)81014-F

Arachidonic acid epoxygenase. Stereochemical analysis of the endogenous epoxyeicosatrienoic acids of human kidney cortex

Armando Karara, Elizabeth Dishman, Harry Jacobson, J. R. Falck, Jorge H. Capdevila

Biochemistry

Research output: Contribution to journal › Article › peer-review

70 Scopus citations

Abstract

Mass spectral and Chromatographic analysis demonstrates the presence of 14,15-, 11,12- and 8,9-epoxyeicosatrienoic acids (44%, 33% and 23% of the total, respectively) in human kidney cortex. Chiral analysis of the human renal expoxyeicosatrienoic acids shows the formation of 8,9-, 11,12- and 14,15-epoxyeicosatrienoic acids in a 1:1, 4:1 and 2:1 ratio of antipodes, respectively. These results demonstrate the biosynthetic origin of the human kidney 11,12- and 14,15-epoxyeicosatrienoic acids and suggest a role for renal cytochrome P-450 in the bioactivation of endogenous pools of arachidonic acid.

Original language	English (US)
Pages (from-to)	227-230
Number of pages	4
Journal	FEBS Letters
Volume	268
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(90)81014-F
State	Published - Jul 30 1990

Keywords

Arachidonic acid
Cytochrome P-450
Epoxyeicosatrienoic acid
Epoxygenase

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(90)81014-F

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title = "Arachidonic acid epoxygenase. Stereochemical analysis of the endogenous epoxyeicosatrienoic acids of human kidney cortex",

abstract = "Mass spectral and Chromatographic analysis demonstrates the presence of 14,15-, 11,12- and 8,9-epoxyeicosatrienoic acids (44%, 33% and 23% of the total, respectively) in human kidney cortex. Chiral analysis of the human renal expoxyeicosatrienoic acids shows the formation of 8,9-, 11,12- and 14,15-epoxyeicosatrienoic acids in a 1:1, 4:1 and 2:1 ratio of antipodes, respectively. These results demonstrate the biosynthetic origin of the human kidney 11,12- and 14,15-epoxyeicosatrienoic acids and suggest a role for renal cytochrome P-450 in the bioactivation of endogenous pools of arachidonic acid.",

keywords = "Arachidonic acid, Cytochrome P-450, Epoxyeicosatrienoic acid, Epoxygenase",

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AU - Karara, Armando

AU - Dishman, Elizabeth

AU - Jacobson, Harry

AU - Falck, J. R.

AU - Capdevila, Jorge H.

PY - 1990/7/30

Y1 - 1990/7/30

N2 - Mass spectral and Chromatographic analysis demonstrates the presence of 14,15-, 11,12- and 8,9-epoxyeicosatrienoic acids (44%, 33% and 23% of the total, respectively) in human kidney cortex. Chiral analysis of the human renal expoxyeicosatrienoic acids shows the formation of 8,9-, 11,12- and 14,15-epoxyeicosatrienoic acids in a 1:1, 4:1 and 2:1 ratio of antipodes, respectively. These results demonstrate the biosynthetic origin of the human kidney 11,12- and 14,15-epoxyeicosatrienoic acids and suggest a role for renal cytochrome P-450 in the bioactivation of endogenous pools of arachidonic acid.

AB - Mass spectral and Chromatographic analysis demonstrates the presence of 14,15-, 11,12- and 8,9-epoxyeicosatrienoic acids (44%, 33% and 23% of the total, respectively) in human kidney cortex. Chiral analysis of the human renal expoxyeicosatrienoic acids shows the formation of 8,9-, 11,12- and 14,15-epoxyeicosatrienoic acids in a 1:1, 4:1 and 2:1 ratio of antipodes, respectively. These results demonstrate the biosynthetic origin of the human kidney 11,12- and 14,15-epoxyeicosatrienoic acids and suggest a role for renal cytochrome P-450 in the bioactivation of endogenous pools of arachidonic acid.

KW - Arachidonic acid

KW - Cytochrome P-450

KW - Epoxyeicosatrienoic acid

KW - Epoxygenase

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Arachidonic acid epoxygenase. Stereochemical analysis of the endogenous epoxyeicosatrienoic acids of human kidney cortex

Abstract

Keywords

ASJC Scopus subject areas

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