Arginine-vasopressin increases the release of ET-1 into perfusate of rat mesenteric artery

Y. Tomobe, Masashi Yanagisawa, A. Fujimori, T. Masaki, K. Goto

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The regulatory systems of endogenous endothelin-1 (ET-1) production from intact tissues and the effects of produced ET- 1 on vascular tonus by a closed circuit perfusion system of rat mesenteric artery were investigated. It was demonstrated that ET-1 is released from intact mesenteric arterial beds from Wistar rats both under basal conditions and after stimulation with arginine-vasopressin (AVP) (10-10-10-9 M). Furthermore, AVP (10-10-10-9 M) markedly and dose-dependently induced the expression of prepro ET- 1 mRNA in the mesenteric arterial beds.Increased release of ET-l by AVP may contribute to maintaining vascular tonus. Concomitant addition of actynomicin D inhibited the increased expression of prepro ET- 1 mRNA and reduced the amount of immunoreactive ET-1 found during the 6-hour perfusate from AVP (10-10 M) -stimulated and -unstimulated tissues.

Original languageEnglish (US)
Pages (from-to)654-661
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume191
Issue number2
DOIs
StatePublished - Mar 15 1993

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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