Arginine-vasopressin increases the release of ET-1 into perfusate of rat mesenteric artery

Y. Tomobe; Masashi Yanagisawa; A. Fujimori; T. Masaki; K. Goto

doi:10.1006/bbrc.1993.1267

Arginine-vasopressin increases the release of ET-1 into perfusate of rat mesenteric artery

Y. Tomobe, Masashi Yanagisawa, A. Fujimori, T. Masaki, K. Goto

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Abstract

The regulatory systems of endogenous endothelin-1 (ET-1) production from intact tissues and the effects of produced ET- 1 on vascular tonus by a closed circuit perfusion system of rat mesenteric artery were investigated. It was demonstrated that ET-1 is released from intact mesenteric arterial beds from Wistar rats both under basal conditions and after stimulation with arginine-vasopressin (AVP) (10^-10-10^-9 M). Furthermore, AVP (10^-10-10^-9 M) markedly and dose-dependently induced the expression of prepro ET- 1 mRNA in the mesenteric arterial beds.Increased release of ET-l by AVP may contribute to maintaining vascular tonus. Concomitant addition of actynomicin D inhibited the increased expression of prepro ET- 1 mRNA and reduced the amount of immunoreactive ET-1 found during the 6-hour perfusate from AVP (10^-10 M) -stimulated and -unstimulated tissues.

Original language	English (US)
Pages (from-to)	654-661
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	191
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1993.1267
State	Published - Mar 15 1993

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Arginine-vasopressin increases the release of ET-1 into perfusate of rat mesenteric artery",

abstract = "The regulatory systems of endogenous endothelin-1 (ET-1) production from intact tissues and the effects of produced ET- 1 on vascular tonus by a closed circuit perfusion system of rat mesenteric artery were investigated. It was demonstrated that ET-1 is released from intact mesenteric arterial beds from Wistar rats both under basal conditions and after stimulation with arginine-vasopressin (AVP) (10-10-10-9 M). Furthermore, AVP (10-10-10-9 M) markedly and dose-dependently induced the expression of prepro ET- 1 mRNA in the mesenteric arterial beds.Increased release of ET-l by AVP may contribute to maintaining vascular tonus. Concomitant addition of actynomicin D inhibited the increased expression of prepro ET- 1 mRNA and reduced the amount of immunoreactive ET-1 found during the 6-hour perfusate from AVP (10-10 M) -stimulated and -unstimulated tissues.",

author = "Y. Tomobe and Masashi Yanagisawa and A. Fujimori and T. Masaki and K. Goto",

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T1 - Arginine-vasopressin increases the release of ET-1 into perfusate of rat mesenteric artery

AU - Tomobe, Y.

AU - Yanagisawa, Masashi

AU - Fujimori, A.

AU - Masaki, T.

AU - Goto, K.

PY - 1993/3/15

Y1 - 1993/3/15

N2 - The regulatory systems of endogenous endothelin-1 (ET-1) production from intact tissues and the effects of produced ET- 1 on vascular tonus by a closed circuit perfusion system of rat mesenteric artery were investigated. It was demonstrated that ET-1 is released from intact mesenteric arterial beds from Wistar rats both under basal conditions and after stimulation with arginine-vasopressin (AVP) (10-10-10-9 M). Furthermore, AVP (10-10-10-9 M) markedly and dose-dependently induced the expression of prepro ET- 1 mRNA in the mesenteric arterial beds.Increased release of ET-l by AVP may contribute to maintaining vascular tonus. Concomitant addition of actynomicin D inhibited the increased expression of prepro ET- 1 mRNA and reduced the amount of immunoreactive ET-1 found during the 6-hour perfusate from AVP (10-10 M) -stimulated and -unstimulated tissues.

AB - The regulatory systems of endogenous endothelin-1 (ET-1) production from intact tissues and the effects of produced ET- 1 on vascular tonus by a closed circuit perfusion system of rat mesenteric artery were investigated. It was demonstrated that ET-1 is released from intact mesenteric arterial beds from Wistar rats both under basal conditions and after stimulation with arginine-vasopressin (AVP) (10-10-10-9 M). Furthermore, AVP (10-10-10-9 M) markedly and dose-dependently induced the expression of prepro ET- 1 mRNA in the mesenteric arterial beds.Increased release of ET-l by AVP may contribute to maintaining vascular tonus. Concomitant addition of actynomicin D inhibited the increased expression of prepro ET- 1 mRNA and reduced the amount of immunoreactive ET-1 found during the 6-hour perfusate from AVP (10-10 M) -stimulated and -unstimulated tissues.

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Arginine-vasopressin increases the release of ET-1 into perfusate of rat mesenteric artery

Abstract

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