The regulatory systems of endogenous endothelin-1 (ET-1) production from intact tissues and the effects of produced ET- 1 on vascular tonus by a closed circuit perfusion system of rat mesenteric artery were investigated. It was demonstrated that ET-1 is released from intact mesenteric arterial beds from Wistar rats both under basal conditions and after stimulation with arginine-vasopressin (AVP) (10-10-10-9 M). Furthermore, AVP (10-10-10-9 M) markedly and dose-dependently induced the expression of prepro ET- 1 mRNA in the mesenteric arterial beds.Increased release of ET-l by AVP may contribute to maintaining vascular tonus. Concomitant addition of actynomicin D inhibited the increased expression of prepro ET- 1 mRNA and reduced the amount of immunoreactive ET-1 found during the 6-hour perfusate from AVP (10-10 M) -stimulated and -unstimulated tissues.
|Original language||English (US)|
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Mar 15 1993|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology