ARH is a modular adaptor protein that interacts with the LDL receptor, clathrin, and AP-2

Guocheng He, Sarita Gupta, Ming Yi, Peter Michaely, Helen H. Hobbs, Jonathan C. Cohen

Research output: Contribution to journalArticle

156 Scopus citations

Abstract

Mutations in the phosphotyrosine binding domain protein ARH cause autosomal recessive hypercholesterolemia, a disorder caused by defective internalization of low density lipoprotein receptors (LDLR) in the liver. To examine the function of ARH, we used pull-down experiments to test for interactions between ARH, the LDLR, and proteins involved in clathrin-mediated endocytosis. The phosphotyrosine binding domain of ARH interacted with the internalization sequence (NPVY) in the cytoplasmic tail of LDLR in a sequence-specific manner. Mutations in the NPVY sequence that were previously shown to decrease LDLR internalization abolished in vitro binding to ARH. Recombinant ARH bound purified bovine clathrin with high affinity (KD, ∼44 nM). The interaction between ARH and clathrin was mapped to a canonical clathrin box sequence (LLDLE) in ARH and to the N-terminal domain of the clathrin heavy chain. A highly conserved 20-amino acid sequence in the C-terminal region of ARH bound the β2-adaptin subunit of AP-2. Mutation of a glutamic acid residue in the appendage domain of β2-adaptin that is required for interaction with the adapter protein β-arrestin markedly reduced binding to ARH. These data are consistent with the hypothesis that ARH functions as an adaptor protein that couples LDLR to the endocytic machinery.

Original languageEnglish (US)
Pages (from-to)44044-44049
Number of pages6
JournalJournal of Biological Chemistry
Volume277
Issue number46
DOIs
StatePublished - Nov 15 2002

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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