Aromatase Expression in the Hippocampus of AD Patients and 5xFAD Mice

Janine Prange-Kiel, Danuta A. Dudzinski, Felicitas Pröls, Markus Glatzel, Jakob Matschke, Gabriele M. Rune

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Numerous studies show that 17β-estradiol (E2) protects against Alzheimer's disease (AD) induced neurodegeneration. The E2-synthesizing enzyme aromatase is expressed in healthy hippocampi, but although the hippocampus is severely affected in AD, little is known about the expression of hippocampal aromatase in AD. To better understand the role of hippocampal aromatase in AD, we studied its expression in postmortem material from patients with AD and in a mouse model for AD (5xFAD mice). In human hippocampi, aromatase-immunoreactivity was observed in the vast majority of principal neurons and signal quantification revealed higher expression of aromatase protein in AD patients compared to age-and sex-matched controls. The tissue-specific first exons of aromatase I.f, PII, I.3, and I.6 were detected in hippocampi of controls and AD patients by RT-PCR. In contrast, 3-month-old, female 5xFAD mice showed lower expression of aromatase mRNA and protein (measured by qRT-PCR and semiquantitative immunohistochemistry) than WT controls; no such differences were observed in male mice. Our findings stress the importance of hippocampal aromatase expression in neurodegenerative diseases.

Original languageEnglish (US)
Article number9802086
JournalNeural Plasticity
Volume2016
DOIs
StatePublished - 2016

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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    Prange-Kiel, J., Dudzinski, D. A., Pröls, F., Glatzel, M., Matschke, J., & Rune, G. M. (2016). Aromatase Expression in the Hippocampus of AD Patients and 5xFAD Mice. Neural Plasticity, 2016, [9802086]. https://doi.org/10.1155/2016/9802086