Aromatization of androstenedione by cultured human fibroblasts

H. U. Schweikert, L. Milewich, J. D. Wilson

Research output: Contribution to journalArticle

138 Scopus citations

Abstract

The conversion of [l, 2, 6, 7-3H]androstenedione to [3H]estrone was measured in fibroblast monolayers grown from biopsies of genital and nongenital skins obtained from 15 control subjects, 9 males with developmental defects of the urogenital tract, and 8 patients with hereditary male pseudohermaphroditism. Under the standardized conditions utilized in this study, the rate of estrone formation in the fibroblasts from normal controls varied from <0.2 to 5.5 pmol/100 mg protein/h, and these rates were enhanced by incubation of intact monolayers with choleragen, theophylline, or dexamethasone. Rates of estrone formation were higher in some foreskin strains grown from subjects with developmental defects of the urogenital tract and in strains from scrotum and foreskin of patients with familial incomplete male pseudohermaphroditism, types 1 and 2 than in normal strains or strains from patients with testicular feminization. The meaning of these apparent high rates of estrone formation is unclear.

Original languageEnglish (US)
Pages (from-to)785-795
Number of pages11
JournalJournal of Clinical Endocrinology and Metabolism
Volume43
Issue number4
DOIs
StatePublished - Oct 1976

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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