Arp2/3-independent assembly of actin by Vibrio type III effector VopL

Amy D B Liverman; Hui Chun Cheng; Jennifer E. Trosky; Daisy W. Leung; Melanie L. Yarbrough; Dara L. Burdette; Michael K. Rosen; Kim Orth

doi:10.1073/pnas.0703196104

Arp2/3-independent assembly of actin by Vibrio type III effector VopL

Amy D B Liverman, Hui Chun Cheng, Jennifer E. Trosky, Daisy W. Leung, Melanie L. Yarbrough, Dara L. Burdette, Michael K. Rosen, Kim Orth

Research output: Contribution to journal › Article › peer-review

131 Scopus citations

Abstract

Microbial pathogens use a variety of mechanisms to disrupt the actin cytoskeleton during infection. Vibrio parahaemolyticus (V. para) is a Gram-negative bacterium that causes gastroenteritis, and new pandemic strains are emerging throughout the world. Analysis of the V. para genome revealed a type III secretion system effector, VopL, encoding three Wiskott-Aldrich homology 2 domains that are interspersed with three proline-rich motifs. Infection of HeLa cells with V. para induces the formation of long actin fibers in a VopL-dependent manner. Transfection of VopL promotes the assembly of actin stress fibers. In vitro, recombinant VopL potently induces assembly of actin filaments that grow at their barbed ends, independent of eukaryotic factors. Vibrio VopL is predicted to be a bacterial virulence factor that disrupts actin homeostasis during an enteric infection of the host.

Original language	English (US)
Pages (from-to)	17117-17122
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	104
Issue number	43
DOIs	https://doi.org/10.1073/pnas.0703196104
State	Published - Oct 23 2007

Keywords

Actin assembly
Microbial pathogenesis
Stress fibers
Virulence
WH2 domains

ASJC Scopus subject areas

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title = "Arp2/3-independent assembly of actin by Vibrio type III effector VopL",

abstract = "Microbial pathogens use a variety of mechanisms to disrupt the actin cytoskeleton during infection. Vibrio parahaemolyticus (V. para) is a Gram-negative bacterium that causes gastroenteritis, and new pandemic strains are emerging throughout the world. Analysis of the V. para genome revealed a type III secretion system effector, VopL, encoding three Wiskott-Aldrich homology 2 domains that are interspersed with three proline-rich motifs. Infection of HeLa cells with V. para induces the formation of long actin fibers in a VopL-dependent manner. Transfection of VopL promotes the assembly of actin stress fibers. In vitro, recombinant VopL potently induces assembly of actin filaments that grow at their barbed ends, independent of eukaryotic factors. Vibrio VopL is predicted to be a bacterial virulence factor that disrupts actin homeostasis during an enteric infection of the host.",

keywords = "Actin assembly, Microbial pathogenesis, Stress fibers, Virulence, WH2 domains",

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year = "2007",

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doi = "10.1073/pnas.0703196104",

language = "English (US)",

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T1 - Arp2/3-independent assembly of actin by Vibrio type III effector VopL

AU - Liverman, Amy D B

AU - Cheng, Hui Chun

AU - Trosky, Jennifer E.

AU - Leung, Daisy W.

AU - Yarbrough, Melanie L.

AU - Burdette, Dara L.

AU - Rosen, Michael K.

AU - Orth, Kim

PY - 2007/10/23

Y1 - 2007/10/23

N2 - Microbial pathogens use a variety of mechanisms to disrupt the actin cytoskeleton during infection. Vibrio parahaemolyticus (V. para) is a Gram-negative bacterium that causes gastroenteritis, and new pandemic strains are emerging throughout the world. Analysis of the V. para genome revealed a type III secretion system effector, VopL, encoding three Wiskott-Aldrich homology 2 domains that are interspersed with three proline-rich motifs. Infection of HeLa cells with V. para induces the formation of long actin fibers in a VopL-dependent manner. Transfection of VopL promotes the assembly of actin stress fibers. In vitro, recombinant VopL potently induces assembly of actin filaments that grow at their barbed ends, independent of eukaryotic factors. Vibrio VopL is predicted to be a bacterial virulence factor that disrupts actin homeostasis during an enteric infection of the host.

AB - Microbial pathogens use a variety of mechanisms to disrupt the actin cytoskeleton during infection. Vibrio parahaemolyticus (V. para) is a Gram-negative bacterium that causes gastroenteritis, and new pandemic strains are emerging throughout the world. Analysis of the V. para genome revealed a type III secretion system effector, VopL, encoding three Wiskott-Aldrich homology 2 domains that are interspersed with three proline-rich motifs. Infection of HeLa cells with V. para induces the formation of long actin fibers in a VopL-dependent manner. Transfection of VopL promotes the assembly of actin stress fibers. In vitro, recombinant VopL potently induces assembly of actin filaments that grow at their barbed ends, independent of eukaryotic factors. Vibrio VopL is predicted to be a bacterial virulence factor that disrupts actin homeostasis during an enteric infection of the host.

KW - Actin assembly

KW - Microbial pathogenesis

KW - Stress fibers

KW - Virulence

KW - WH2 domains

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

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ER -

Arp2/3-independent assembly of actin by Vibrio type III effector VopL

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Keywords

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