Ars2 Links the Nuclear Cap-Binding Complex to RNA Interference and Cell Proliferation

Joshua J. Gruber, D. Steven Zatechka, Leah R. Sabin, Jeongsik Yong, Julian J. Lum, Mei Kong, Wei Xing Zong, Zhenxi Zhang, Chi Kong Lau, Jason Rawlings, Sara Cherry, James N. Ihle, Gideon Dreyfuss, Craig B. Thompson

Research output: Contribution to journalArticlepeer-review

149 Scopus citations

Abstract

Here we identify a component of the nuclear RNA cap-binding complex (CBC), Ars2, that is important for miRNA biogenesis and critical for cell proliferation. Unlike other components of the CBC, Ars2 expression is linked to the proliferative state of the cell. Deletion of Ars2 is developmentally lethal, and deletion in adult mice led to bone marrow failure whereas parenchymal organs composed of nonproliferating cells were unaffected. Depletion of Ars2 or CBP80 from proliferating cells impaired miRNA-mediated repression and led to alterations in primary miRNA processing in the nucleus. Ars2 depletion also reduced the levels of several miRNAs, including miR-21, let-7, and miR-155, that are implicated in cellular transformation. These findings provide evidence for a role for Ars2 in RNA interference regulation during cell proliferation.

Original languageEnglish (US)
Pages (from-to)328-339
Number of pages12
JournalCell
Volume138
Issue number2
DOIs
StatePublished - Jul 23 2009
Externally publishedYes

Keywords

  • CELLCYCLE
  • RNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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