Ascl 1 defines sequentially generated lineage-resricted neuronal and oligodendrocyte precursor cells in the spinal cord

James Battiste, Amy W. Helms, Euiseok J. Kim, Trisha K. Savage, Diane C. Lagace, Chitra D. Mandyam, Amelia J. Eisch, Goichi Miyoshi, Jane E. Johnson

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

The neural basic helix-loop-helix transcription factor Ascl1 (previously Mash1) is present in ventricularzone cells in restricted domains throughout the developing nervous system. This study uses genetic fate mapping to define the stage and neural lineages in the developing spinal cord that are derived from Ascl1-expressing cells. We find that Ascl1 is present in progenitors to both neurons and oligodendrocytes, but not astrocytes. Temporal control of the fate-mapping paradigm reveals rapid cell-cycle exit and differentiation of Ascl1-expressing cells. At embryonic day 11, Ascl1 identifies neuronal-restricted precursor cells that become dorsal horn neurons in the superficial laminae. By contrast, at embryonic day 16, Ascl1 identifies of oligodendrocyte-restricted precursor cells that distribute throughout the spinal cord. These data demonstrate that sequentially generated Ascl1-expressing progenitors give rise first to dorsal horn interneurons and subsequently to late-born oligodendrocytes. Furthermore, Ascl1-null cells in the spinal cord have a diminished capacity to undergo neuronal differentiation, with a subset of these cells retaining characteristics of immature glial cells.

Original languageEnglish (US)
Pages (from-to)285-293
Number of pages9
JournalDevelopment
Volume134
Issue number2
DOIs
StatePublished - Jan 2007

Fingerprint

Oligodendroglia
Spinal Cord
Basic Helix-Loop-Helix Transcription Factors
Posterior Horn Cells
Null Lymphocytes
Interneurons
Neuroglia
Astrocytes
Nervous System
Cell Differentiation
Cell Cycle
Neurons

Keywords

  • bHLH transcription factor
  • In vivo genetic fate mapping
  • Mash1 (Ascl 1)
  • Mouse
  • Spinal cord development

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Ascl 1 defines sequentially generated lineage-resricted neuronal and oligodendrocyte precursor cells in the spinal cord. / Battiste, James; Helms, Amy W.; Kim, Euiseok J.; Savage, Trisha K.; Lagace, Diane C.; Mandyam, Chitra D.; Eisch, Amelia J.; Miyoshi, Goichi; Johnson, Jane E.

In: Development, Vol. 134, No. 2, 01.2007, p. 285-293.

Research output: Contribution to journalArticle

Battiste, James ; Helms, Amy W. ; Kim, Euiseok J. ; Savage, Trisha K. ; Lagace, Diane C. ; Mandyam, Chitra D. ; Eisch, Amelia J. ; Miyoshi, Goichi ; Johnson, Jane E. / Ascl 1 defines sequentially generated lineage-resricted neuronal and oligodendrocyte precursor cells in the spinal cord. In: Development. 2007 ; Vol. 134, No. 2. pp. 285-293.
@article{cbd630986310493f8b7cfa9633c1203e,
title = "Ascl 1 defines sequentially generated lineage-resricted neuronal and oligodendrocyte precursor cells in the spinal cord",
abstract = "The neural basic helix-loop-helix transcription factor Ascl1 (previously Mash1) is present in ventricularzone cells in restricted domains throughout the developing nervous system. This study uses genetic fate mapping to define the stage and neural lineages in the developing spinal cord that are derived from Ascl1-expressing cells. We find that Ascl1 is present in progenitors to both neurons and oligodendrocytes, but not astrocytes. Temporal control of the fate-mapping paradigm reveals rapid cell-cycle exit and differentiation of Ascl1-expressing cells. At embryonic day 11, Ascl1 identifies neuronal-restricted precursor cells that become dorsal horn neurons in the superficial laminae. By contrast, at embryonic day 16, Ascl1 identifies of oligodendrocyte-restricted precursor cells that distribute throughout the spinal cord. These data demonstrate that sequentially generated Ascl1-expressing progenitors give rise first to dorsal horn interneurons and subsequently to late-born oligodendrocytes. Furthermore, Ascl1-null cells in the spinal cord have a diminished capacity to undergo neuronal differentiation, with a subset of these cells retaining characteristics of immature glial cells.",
keywords = "bHLH transcription factor, In vivo genetic fate mapping, Mash1 (Ascl 1), Mouse, Spinal cord development",
author = "James Battiste and Helms, {Amy W.} and Kim, {Euiseok J.} and Savage, {Trisha K.} and Lagace, {Diane C.} and Mandyam, {Chitra D.} and Eisch, {Amelia J.} and Goichi Miyoshi and Johnson, {Jane E.}",
year = "2007",
month = "1",
doi = "10.1242/dev.02727",
language = "English (US)",
volume = "134",
pages = "285--293",
journal = "Development (Cambridge)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "2",

}

TY - JOUR

T1 - Ascl 1 defines sequentially generated lineage-resricted neuronal and oligodendrocyte precursor cells in the spinal cord

AU - Battiste, James

AU - Helms, Amy W.

AU - Kim, Euiseok J.

AU - Savage, Trisha K.

AU - Lagace, Diane C.

AU - Mandyam, Chitra D.

AU - Eisch, Amelia J.

AU - Miyoshi, Goichi

AU - Johnson, Jane E.

PY - 2007/1

Y1 - 2007/1

N2 - The neural basic helix-loop-helix transcription factor Ascl1 (previously Mash1) is present in ventricularzone cells in restricted domains throughout the developing nervous system. This study uses genetic fate mapping to define the stage and neural lineages in the developing spinal cord that are derived from Ascl1-expressing cells. We find that Ascl1 is present in progenitors to both neurons and oligodendrocytes, but not astrocytes. Temporal control of the fate-mapping paradigm reveals rapid cell-cycle exit and differentiation of Ascl1-expressing cells. At embryonic day 11, Ascl1 identifies neuronal-restricted precursor cells that become dorsal horn neurons in the superficial laminae. By contrast, at embryonic day 16, Ascl1 identifies of oligodendrocyte-restricted precursor cells that distribute throughout the spinal cord. These data demonstrate that sequentially generated Ascl1-expressing progenitors give rise first to dorsal horn interneurons and subsequently to late-born oligodendrocytes. Furthermore, Ascl1-null cells in the spinal cord have a diminished capacity to undergo neuronal differentiation, with a subset of these cells retaining characteristics of immature glial cells.

AB - The neural basic helix-loop-helix transcription factor Ascl1 (previously Mash1) is present in ventricularzone cells in restricted domains throughout the developing nervous system. This study uses genetic fate mapping to define the stage and neural lineages in the developing spinal cord that are derived from Ascl1-expressing cells. We find that Ascl1 is present in progenitors to both neurons and oligodendrocytes, but not astrocytes. Temporal control of the fate-mapping paradigm reveals rapid cell-cycle exit and differentiation of Ascl1-expressing cells. At embryonic day 11, Ascl1 identifies neuronal-restricted precursor cells that become dorsal horn neurons in the superficial laminae. By contrast, at embryonic day 16, Ascl1 identifies of oligodendrocyte-restricted precursor cells that distribute throughout the spinal cord. These data demonstrate that sequentially generated Ascl1-expressing progenitors give rise first to dorsal horn interneurons and subsequently to late-born oligodendrocytes. Furthermore, Ascl1-null cells in the spinal cord have a diminished capacity to undergo neuronal differentiation, with a subset of these cells retaining characteristics of immature glial cells.

KW - bHLH transcription factor

KW - In vivo genetic fate mapping

KW - Mash1 (Ascl 1)

KW - Mouse

KW - Spinal cord development

UR - http://www.scopus.com/inward/record.url?scp=33846977750&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846977750&partnerID=8YFLogxK

U2 - 10.1242/dev.02727

DO - 10.1242/dev.02727

M3 - Article

C2 - 17166924

AN - SCOPUS:33846977750

VL - 134

SP - 285

EP - 293

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

IS - 2

ER -