Ascl1 (Mash1) lineage cells contribute to discrete cell populations in CNS architecture

Euiseok J. Kim, James Battiste, Yasushi Nakagawa, Jane E. Johnson

Research output: Contribution to journalArticlepeer-review

101 Scopus citations

Abstract

Ascl1 (previously Mash1) is a bHLH transcription factor essential for neuronal differentiation and specification in the nervous system. Although it has been studied for its role in several neural lineages, the full complement of lineages arising from Ascl1 progenitor cells remains unknown. Using an inducible Cre-flox genetic fate-mapping strategy, Ascl1 lineages were determined throughout the brain. Ascl1 is present in proliferating progenitor cells but these cells are actively differentiating as evidenced by rapid migration out of germinal zones. Ascl1 lineage cells contribute to distinct cell types in each major brain division: the forebrain including the cerebral cortex, olfactory bulb, hippocampus, striatum, hypothalamus, and thalamic nuclei, the midbrain including superior and inferior colliculi, and the hindbrain including Purkinje and deep cerebellar nuclei cells and cells in the trigeminal sensory system. Ascl1 progenitor cells at early stages in each CNS region preferentially become neurons, and at late stages they become oligodendrocytes. In conclusion, Ascl1-expressing progenitor cells in the brain give rise to multiple, but not all, neuronal subtypes and oligodendrocytes depending on the temporal and spatial context, consistent with a broad role in neural differentiation with some subtype specification.

Original languageEnglish (US)
Pages (from-to)595-606
Number of pages12
JournalMolecular and Cellular Neuroscience
Volume38
Issue number4
DOIs
StatePublished - Aug 4 2008

Keywords

  • Ascl1
  • Brain development
  • Brainstem
  • Cerebellum
  • Genetic fate mapping
  • Mash1
  • bHLH transcription factor

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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