ASCL1 regulates proliferation of NG2-glia in the embryonic and adult spinal cord

Demetra P. Kelenis, Emma Hart, Morgan Edwards-Fligner, Jane E. Johnson, Tou Yia Vue

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

NG2-glia are highly proliferative oligodendrocyte precursor cells (OPCs) that are widely distributed throughout the central nervous system (CNS). During development, NG2-glia predominantly differentiate into oligodendrocytes (OLs) to myelinate axon fibers, but they can also remain as OPCs persisting into the mature CNS. Interestingly, NG2-glia in the gray matter (GM) are intrinsically different from those in the white matter (WM) in terms of proliferation, differentiation, gene expression, and electrophysiological properties. Here we investigate the role of the transcriptional regulator, ASCL1, in controlling NG2-glia distribution and development in the GM and WM. In the spinal cord, ASCL1 levels are higher in WM NG2-glia than those in the GM. This differential level of ASCL1 in WM and GM NG2-glia is maintained into adult stages. Long-term clonal lineage analysis reveals that the progeny of single ASCL1+ oligodendrocyte progenitors (OLPs) and NG2-glia are primarily restricted to the GM or WM, even though they undergo extensive proliferation to give rise to large clusters of OLs in the postnatal spinal cord. Conditional deletion of Ascl1 specifically in NG2-glia in the embryonic or adult spinal cord resulted in a significant reduction in the proliferation but not differentiation of these cells. These findings illustrate that ASCL1 is an intrinsic regulator of the proliferative property of NG2-glia in the CNS.

Original languageEnglish (US)
JournalGLIA
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Neuroglia
Spinal Cord
Oligodendroglia
Central Nervous System
Axons
Cell Differentiation
Gray Matter
White Matter
Gene Expression

Keywords

  • ASCL1
  • NG2-glia
  • Oligodendrogenesis
  • OPCs
  • Proliferation
  • Spinal cord

ASJC Scopus subject areas

  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

ASCL1 regulates proliferation of NG2-glia in the embryonic and adult spinal cord. / Kelenis, Demetra P.; Hart, Emma; Edwards-Fligner, Morgan; Johnson, Jane E.; Vue, Tou Yia.

In: GLIA, 01.01.2018.

Research output: Contribution to journalArticle

Kelenis, Demetra P. ; Hart, Emma ; Edwards-Fligner, Morgan ; Johnson, Jane E. ; Vue, Tou Yia. / ASCL1 regulates proliferation of NG2-glia in the embryonic and adult spinal cord. In: GLIA. 2018.
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