Assay of a ribonuclease that preferentially hydrolyses mRNAs containing cytokine-derived UA-rich instability sequences

Bruce Beutler, Patricia Thompson, Jennifer Keyes, Kathleen Hagerty, David Crawford

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

mRNA molecules encoding a number of inflammatory cytokines, as well as certain proto-oncogenes, contain a conserved UA-exclusive sequence in the 3′ untranslated region that confers message instability in vivo. This sequence may comprise a critical regulatory element, governing the level of these mRNA molecules, and determining the efficiency with which they are translated. Through the use of a double-label RNAse assay, we have determined that lysates prepared from mouse macrophages selectively degrade mRNA molecules containing the 3′ untranslated UA sequence found in the mRNA encoding human cachectin/TNF. The degree of instability is dependent upon the number of copies of inserted UA sequence present in the target mRNA molecule (a Xenopus β-globin mRNA). mRNAs containing randomly generated UA sequences are more labile than unmodified globin mRNA, but less susceptible to degradation than mRNAs containing the authentic cachectin-derived sequence. mRNA molecules containing synthetic UG-exclusive sequences are normally stable or protected in vitro. The destruction of UA-containing mRNA is inhibited by random adenylate/uridilate copolymers, but not by guanylate/uridilate copolymers. Boiling or proteinase K treatment destroys the selective nucleolytic activity of macrophage lysates. We propose that the nuclease measured here may serve to regulate cellular levels of mRNA molecules encoding cachectin, other inflammatory cytokines, and certain proto-oncogene products.

Original languageEnglish (US)
Pages (from-to)973-980
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume152
Issue number3
DOIs
StatePublished - May 16 1988

Fingerprint

Ribonucleases
Hydrolysis
Assays
Cytokines
Messenger RNA
Molecules
Globins
Proto-Oncogenes
Tumor Necrosis Factor-alpha
Macrophages
Copolymers
Endopeptidase K
Oncogene Proteins
RNA Stability
3' Untranslated Regions
Xenopus
Boiling liquids
Labels
Degradation

Keywords

  • 3′-untranslated region
  • Cachectin
  • macrophage
  • message stability
  • mRNA
  • ribonuclease
  • TTATTTAT sequence
  • tumor necrosis factor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Assay of a ribonuclease that preferentially hydrolyses mRNAs containing cytokine-derived UA-rich instability sequences. / Beutler, Bruce; Thompson, Patricia; Keyes, Jennifer; Hagerty, Kathleen; Crawford, David.

In: Biochemical and Biophysical Research Communications, Vol. 152, No. 3, 16.05.1988, p. 973-980.

Research output: Contribution to journalArticle

Beutler, Bruce ; Thompson, Patricia ; Keyes, Jennifer ; Hagerty, Kathleen ; Crawford, David. / Assay of a ribonuclease that preferentially hydrolyses mRNAs containing cytokine-derived UA-rich instability sequences. In: Biochemical and Biophysical Research Communications. 1988 ; Vol. 152, No. 3. pp. 973-980.
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