Assay of urinary oxalate

Six methodologies compared

J. E. Zerwekh, E. Drake, J. Gregory, D. Griffith, A. F. Hofmann, M. Menon, C. Y. Pak

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

To assess how well results by different methods for urinary oxalate determinations agree with each other in a clinical setting, we compared six different assays: Hodgkinson and Williams, enzymatic, modified Hodgkinson and Williams, gas chromatography, ion chromatography, and 'high-pressure' liquid chromatography. For the entire group of samples, the mean value by each method agreed relatively closely, although the enzymatic procedure produced a somewhat higher value. All six methods had large coefficients of variation within (8-58%) and between (15-88%) assays. In addition, analytical recovery by most assays was more than 100% of the added oxalate. Analytical recovery of 10 μg of oxalate added per milliliter of urine specimen ranged from 86 to 237%; for 20 μg/mL it was 83 to 320%. Thus for the six methods evaluated, no single method appeared to be superior to the others.

Original languageEnglish (US)
Pages (from-to)1977-1980
Number of pages4
JournalClinical Chemistry
Volume29
Issue number11
StatePublished - 1983

Fingerprint

Oxalates
Assays
High pressure liquid chromatography
Ion chromatography
Recovery
Gas chromatography
Gas Chromatography
Chromatography
High Pressure Liquid Chromatography
Urine
Ions

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Zerwekh, J. E., Drake, E., Gregory, J., Griffith, D., Hofmann, A. F., Menon, M., & Pak, C. Y. (1983). Assay of urinary oxalate: Six methodologies compared. Clinical Chemistry, 29(11), 1977-1980.

Assay of urinary oxalate : Six methodologies compared. / Zerwekh, J. E.; Drake, E.; Gregory, J.; Griffith, D.; Hofmann, A. F.; Menon, M.; Pak, C. Y.

In: Clinical Chemistry, Vol. 29, No. 11, 1983, p. 1977-1980.

Research output: Contribution to journalArticle

Zerwekh, JE, Drake, E, Gregory, J, Griffith, D, Hofmann, AF, Menon, M & Pak, CY 1983, 'Assay of urinary oxalate: Six methodologies compared', Clinical Chemistry, vol. 29, no. 11, pp. 1977-1980.
Zerwekh JE, Drake E, Gregory J, Griffith D, Hofmann AF, Menon M et al. Assay of urinary oxalate: Six methodologies compared. Clinical Chemistry. 1983;29(11):1977-1980.
Zerwekh, J. E. ; Drake, E. ; Gregory, J. ; Griffith, D. ; Hofmann, A. F. ; Menon, M. ; Pak, C. Y. / Assay of urinary oxalate : Six methodologies compared. In: Clinical Chemistry. 1983 ; Vol. 29, No. 11. pp. 1977-1980.
@article{b25035ffe4b94f2cb51ae27b91031e4d,
title = "Assay of urinary oxalate: Six methodologies compared",
abstract = "To assess how well results by different methods for urinary oxalate determinations agree with each other in a clinical setting, we compared six different assays: Hodgkinson and Williams, enzymatic, modified Hodgkinson and Williams, gas chromatography, ion chromatography, and 'high-pressure' liquid chromatography. For the entire group of samples, the mean value by each method agreed relatively closely, although the enzymatic procedure produced a somewhat higher value. All six methods had large coefficients of variation within (8-58{\%}) and between (15-88{\%}) assays. In addition, analytical recovery by most assays was more than 100{\%} of the added oxalate. Analytical recovery of 10 μg of oxalate added per milliliter of urine specimen ranged from 86 to 237{\%}; for 20 μg/mL it was 83 to 320{\%}. Thus for the six methods evaluated, no single method appeared to be superior to the others.",
author = "Zerwekh, {J. E.} and E. Drake and J. Gregory and D. Griffith and Hofmann, {A. F.} and M. Menon and Pak, {C. Y.}",
year = "1983",
language = "English (US)",
volume = "29",
pages = "1977--1980",
journal = "Clinical Chemistry",
issn = "0009-9147",
publisher = "American Association for Clinical Chemistry Inc.",
number = "11",

}

TY - JOUR

T1 - Assay of urinary oxalate

T2 - Six methodologies compared

AU - Zerwekh, J. E.

AU - Drake, E.

AU - Gregory, J.

AU - Griffith, D.

AU - Hofmann, A. F.

AU - Menon, M.

AU - Pak, C. Y.

PY - 1983

Y1 - 1983

N2 - To assess how well results by different methods for urinary oxalate determinations agree with each other in a clinical setting, we compared six different assays: Hodgkinson and Williams, enzymatic, modified Hodgkinson and Williams, gas chromatography, ion chromatography, and 'high-pressure' liquid chromatography. For the entire group of samples, the mean value by each method agreed relatively closely, although the enzymatic procedure produced a somewhat higher value. All six methods had large coefficients of variation within (8-58%) and between (15-88%) assays. In addition, analytical recovery by most assays was more than 100% of the added oxalate. Analytical recovery of 10 μg of oxalate added per milliliter of urine specimen ranged from 86 to 237%; for 20 μg/mL it was 83 to 320%. Thus for the six methods evaluated, no single method appeared to be superior to the others.

AB - To assess how well results by different methods for urinary oxalate determinations agree with each other in a clinical setting, we compared six different assays: Hodgkinson and Williams, enzymatic, modified Hodgkinson and Williams, gas chromatography, ion chromatography, and 'high-pressure' liquid chromatography. For the entire group of samples, the mean value by each method agreed relatively closely, although the enzymatic procedure produced a somewhat higher value. All six methods had large coefficients of variation within (8-58%) and between (15-88%) assays. In addition, analytical recovery by most assays was more than 100% of the added oxalate. Analytical recovery of 10 μg of oxalate added per milliliter of urine specimen ranged from 86 to 237%; for 20 μg/mL it was 83 to 320%. Thus for the six methods evaluated, no single method appeared to be superior to the others.

UR - http://www.scopus.com/inward/record.url?scp=0020512125&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020512125&partnerID=8YFLogxK

M3 - Article

VL - 29

SP - 1977

EP - 1980

JO - Clinical Chemistry

JF - Clinical Chemistry

SN - 0009-9147

IS - 11

ER -