Assay of urinary oxalate: Six methodologies compared

J. E. Zerwekh, E. Drake, J. Gregory, D. Griffith, A. F. Hofmann, M. Menon, C. Y. Pak

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

To assess how well results by different methods for urinary oxalate determinations agree with each other in a clinical setting, we compared six different assays: Hodgkinson and Williams, enzymatic, modified Hodgkinson and Williams, gas chromatography, ion chromatography, and 'high-pressure' liquid chromatography. For the entire group of samples, the mean value by each method agreed relatively closely, although the enzymatic procedure produced a somewhat higher value. All six methods had large coefficients of variation within (8-58%) and between (15-88%) assays. In addition, analytical recovery by most assays was more than 100% of the added oxalate. Analytical recovery of 10 μg of oxalate added per milliliter of urine specimen ranged from 86 to 237%; for 20 μg/mL it was 83 to 320%. Thus for the six methods evaluated, no single method appeared to be superior to the others.

Original languageEnglish (US)
Pages (from-to)1977-1980
Number of pages4
JournalClinical chemistry
Volume29
Issue number11
StatePublished - Dec 27 1983

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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    Zerwekh, J. E., Drake, E., Gregory, J., Griffith, D., Hofmann, A. F., Menon, M., & Pak, C. Y. (1983). Assay of urinary oxalate: Six methodologies compared. Clinical chemistry, 29(11), 1977-1980.