Assay Principles in CLinical Pathology

L. J. Kricka, J. Y. Park

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A wide range of analytic methods are currently employed in cLinical laboratory analyses. These range from simple one-step mix and measure colorimetric assays to more complex multistep assays that combine a separation and a detection method. Different regions of the electromagnetic spectrum have been exploited in analysis ranging from infrared (e.g., noninvasive blood oxygen measurement) to the ultraviolet (e.g., coupled enzyme assays based on nicotinamide adenine dinucleotide measurement). Based on measurements in the electromagnetic spectrum, cLinically significant biomolecules, electrolytes, and therapeutic compounds can be identified and quantified. This article surveys the scope of analytic techniques used in modern-day cLinical laboratories.

Original languageEnglish (US)
Title of host publicationPathobiology of Human Disease
Subtitle of host publicationA Dynamic Encyclopedia of Disease Mechanisms
PublisherElsevier Inc.
Pages3207-3221
Number of pages15
ISBN (Electronic)9780123864567
ISBN (Print)9780123864574
DOIs
StatePublished - Jan 1 2014

Keywords

  • Beer's law
  • Chemiluminescence
  • Electromagnetic spectrum
  • Fluorescence
  • Immunoassay
  • Light scattering
  • Nanoparticles
  • Photobleaching
  • Spectrophotometry
  • Ultraviolet

ASJC Scopus subject areas

  • Medicine(all)

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