Assays for investigating transcription by RNA polymerase II in vitro

Daniel Reines, Arik Dvir, Joan Weliky Conaway, Ronald C. Conaway

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

With the availability of the general initiation factors (TFIIB, TFIID, TFIIE, TFIIF, and TFIIH), it is now possible to investigate aspects of the mechanism of eukaryotic messenger RNA synthesis in purified, reconstituted RNA polymerase II transcription systems. Rapid progress in these investigations has been spurred by use of a growing number of assays that are proving valuable not only for dissecting the molecular mechanisms of transcription initiation and elongation by RNA polymerase II, but also for identifying and purifying novel transcription factors that regulate polymerase activity. Here we describe a variety of these assays and discuss their utility in the analysis of transcription by RNA polymerase II.

Original languageEnglish (US)
Pages (from-to)192-202
Number of pages11
JournalMethods: A Companion to Methods in Enzymology
Volume12
Issue number3
DOIs
StatePublished - Jul 1997
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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