Assays of recombinant adenylyl cyclases expressed in Sf9 cells.

Research output: Contribution to journalArticle

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Abstract

This chapter outlines procedures for the expression of mammalian membrane-bound adenylyl cyclases (AC) in Sf9 cells and subsequent in vitro methods for assessing the activity of these cyclases. Membrane preparations derived from this overexpression system provide homogeneous sources of mammalian AC because AC that are endogenously expressed in Sf9 cells contribute low amounts of activity relative to the overexpressed enzyme. These approaches are applicable to all nine isoforms of mammalian membrane-bound AC isoforms.

Original languageEnglish (US)
Pages (from-to)77-85
Number of pages9
JournalMethods in molecular biology (Clifton, N.J.)
Volume237
StatePublished - 2004

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Sf9 Cells
Adenylyl Cyclases
Membranes
Protein Isoforms
Enzymes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Assays of recombinant adenylyl cyclases expressed in Sf9 cells. / Taussig, Ronald.

In: Methods in molecular biology (Clifton, N.J.), Vol. 237, 2004, p. 77-85.

Research output: Contribution to journalArticle

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