Assessment of circularized E7 RNA, GLUT1, and PD-L1 in anal squamous cell carcinoma

Bahir H. Chamseddin, Eunice E. Lee, Jiwoong Kim, Xiaowei Zhan, Rong Yang, Kathleen M. Murphy, Cheryl Lewis, Gregory A. Hosler, Suntrea T. Hammer, Richard C. Wang

Research output: Contribution to journalArticle

Abstract

Anal squamous cell carcinoma (ASCC) is a rare, potentially fatal malignancy primarily caused by high-risk human papillomaviruses (HPV). The prognostic implication of programmed death-ligand 1 (PD-L1) expression remains controversial, and glucose transporter 1 (GLUT1) expression has never been examined in ASCC. Covalently closed circular RNAs have recently been shown to be widespread in cancers and are proposed to be biomarkers. We discovered HPV16 expresses a circular E7 RNA (circE7) which has not been assessed as a potential biomarker. A retrospective, translational case series at UT Southwestern was conducted to analyze PD-L1, GLUT1, HPV-ISH, and HPV circE7 in relation to the clinical features and overall survival of patients with ASCC. Twenty-two (22) subjects were included in the study. Improved overall survival was predicted by basaloid histology (p = 0.013), PD-L1 expression (p = 0.08), and HPV-ISH positivity (p < 0.001), but not GLUT1 expression. High levels of circE7 by quantitative RT-PCR predicted improved overall survival in ASCC (p = 0.023) and analysis of The Cancer Genome Atlas sequencing from HPV-positive head and neck cancer and cervical cancer suggested high circE7 marked improved survival in 875 subjects (p = 0.074). While our study suggests that circE7 levels correlate with improved survival in ASCC, larger, prospective studies are necessary to confirm the potential role of circE7 as a biomarker.

Original languageEnglish (US)
Pages (from-to)5958-5969
Number of pages12
JournalOncotarget
Volume10
Issue number57
StatePublished - Oct 1 2019

Fingerprint

Facilitative Glucose Transport Proteins
Squamous Cell Carcinoma
RNA
Ligands
Survival
Biomarkers
Neoplasms
Atlases
Head and Neck Neoplasms
circular RNA
Uterine Cervical Neoplasms
Histology
Genome
Prospective Studies
Polymerase Chain Reaction

Keywords

  • Anal squamous cell carcinoma
  • Circular RNA
  • GLUT1
  • Human papillomavirus
  • PD-L1

ASJC Scopus subject areas

  • Oncology

Cite this

Chamseddin, B. H., Lee, E. E., Kim, J., Zhan, X., Yang, R., Murphy, K. M., ... Wang, R. C. (2019). Assessment of circularized E7 RNA, GLUT1, and PD-L1 in anal squamous cell carcinoma. Oncotarget, 10(57), 5958-5969.

Assessment of circularized E7 RNA, GLUT1, and PD-L1 in anal squamous cell carcinoma. / Chamseddin, Bahir H.; Lee, Eunice E.; Kim, Jiwoong; Zhan, Xiaowei; Yang, Rong; Murphy, Kathleen M.; Lewis, Cheryl; Hosler, Gregory A.; Hammer, Suntrea T.; Wang, Richard C.

In: Oncotarget, Vol. 10, No. 57, 01.10.2019, p. 5958-5969.

Research output: Contribution to journalArticle

Chamseddin, BH, Lee, EE, Kim, J, Zhan, X, Yang, R, Murphy, KM, Lewis, C, Hosler, GA, Hammer, ST & Wang, RC 2019, 'Assessment of circularized E7 RNA, GLUT1, and PD-L1 in anal squamous cell carcinoma', Oncotarget, vol. 10, no. 57, pp. 5958-5969.
Chamseddin BH, Lee EE, Kim J, Zhan X, Yang R, Murphy KM et al. Assessment of circularized E7 RNA, GLUT1, and PD-L1 in anal squamous cell carcinoma. Oncotarget. 2019 Oct 1;10(57):5958-5969.
Chamseddin, Bahir H. ; Lee, Eunice E. ; Kim, Jiwoong ; Zhan, Xiaowei ; Yang, Rong ; Murphy, Kathleen M. ; Lewis, Cheryl ; Hosler, Gregory A. ; Hammer, Suntrea T. ; Wang, Richard C. / Assessment of circularized E7 RNA, GLUT1, and PD-L1 in anal squamous cell carcinoma. In: Oncotarget. 2019 ; Vol. 10, No. 57. pp. 5958-5969.
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