Assessment of Posttranslational Modifications of ATG proteins

Y. Xie, R. Kang, D. Tang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Posttranslational modifications (PTMs) increase the functional diversity of proteins and play a key role in many cellular processes. Macroautophagy (hereafter simply referred to as autophagy) is an evolutionarily conserved, lysosome-dependent degradation pathway. This process is finely regulated by autophagy-related (ATG) genes widely conserved among eukaryotes from yeast to mammals. Various PTMs of ATG proteins such as phosphorylation, ubiquitination, and acetylation have been theorized to play a critical role in modulating autophagic processes and activity. In this chapter, we introduce several antibody-based tools (e.g., Western blot, Simple Western™, immunofluorescence, and immunoprecipitation) that are widely used to assess the PTMs of ATG proteins in mammalian cells.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages171-188
Number of pages18
DOIs
StatePublished - 2017

Publication series

NameMethods in Enzymology
Volume587
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Autophagy
  • Autophagy-related protein
  • Immunofluorescence
  • Immunoprecipitation
  • Posttranslational modification
  • Simple Western
  • Western blot

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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