Assessment of requirements for IL-15 and IFN regulatory factors in uterine NK cell differentiation and function during pregnancy

Ali A. Ashkar, Gordon P. Black, Qingxia Wei, Hong He, Luchuan Liang, Judith R. Head, B. Anne Croy

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Abstract

In mouse and human, precursors of NK cell lineage home to decidualizing uteri. To assess the requirement for IL-15, an essential cytokine for NK differentiation in lymphoid tissue, on uterine NK (uNK) cell differentiation, implantation sites from IL-15-/- mice were analyzed histologically. IL-15-/- implantation sites had no uNK cells, no spiral-artery modification, and lacked the decidual integrity found in normal mice. IL-15 -/- recipients of C57BL/6 marrow displayed similar pathology. However, implantation sites from recombination-activating gene-2 -/-γc -/- (alymphoid) recipients of IL-15-/- marrow showed normal uNK cells, modified spiral arteries, and well-developed decidua basalis. Deletion of the IFN-regulatory factor (IRF)-1, but not IRF-2 (factors important in peripheral NK cell differentiation) limited but did not prevent uNK cell development. In situ hybridization localized IRF-1 largely to placental trophoblast cells. IRF-1-/- marrow transplanted into recombination-activating gene-2-/-γ c -/- displayed competence for full uNK cell differentiation. IL-15 mRNA expression at implantation sites of IRF-1 -/- and C57BL/6 was similar, suggesting that, unlike in bone marrow and spleen, IRF-1-/- does not regulate IL-15 in the pregnant uterus. Terminal differentiation of uNK cells was not promoted in pregnant IRF-1 -/- mice by 5-day infusion of murine rIL-15, suggesting that IRF-1 deficiency rather than IL-15 deficiency limits uNK cell differentiation in these mice. Further, IRF-1 regulates placental growth, birth weight, and postnatal growth of offspring. These studies indicate that uNK cell development and maturation share some aspects with NK cell development in other tissues, but also display distinctive tissue-specific regulation.

Original languageEnglish (US)
Pages (from-to)2937-2944
Number of pages8
JournalJournal of Immunology
Volume171
Issue number6
StatePublished - Sep 15 2003

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Interleukin-15
Natural Killer Cells
Cell Differentiation
Pregnancy
Bone Marrow
Genetic Recombination
Uterus
Arteries
Decidua
Trophoblasts
Lymphoid Tissue
Cell Lineage
Growth
Birth Weight
Mental Competency
Genes
In Situ Hybridization
Spleen

ASJC Scopus subject areas

  • Immunology

Cite this

Ashkar, A. A., Black, G. P., Wei, Q., He, H., Liang, L., Head, J. R., & Croy, B. A. (2003). Assessment of requirements for IL-15 and IFN regulatory factors in uterine NK cell differentiation and function during pregnancy. Journal of Immunology, 171(6), 2937-2944.

Assessment of requirements for IL-15 and IFN regulatory factors in uterine NK cell differentiation and function during pregnancy. / Ashkar, Ali A.; Black, Gordon P.; Wei, Qingxia; He, Hong; Liang, Luchuan; Head, Judith R.; Croy, B. Anne.

In: Journal of Immunology, Vol. 171, No. 6, 15.09.2003, p. 2937-2944.

Research output: Contribution to journalArticle

Ashkar, AA, Black, GP, Wei, Q, He, H, Liang, L, Head, JR & Croy, BA 2003, 'Assessment of requirements for IL-15 and IFN regulatory factors in uterine NK cell differentiation and function during pregnancy', Journal of Immunology, vol. 171, no. 6, pp. 2937-2944.
Ashkar, Ali A. ; Black, Gordon P. ; Wei, Qingxia ; He, Hong ; Liang, Luchuan ; Head, Judith R. ; Croy, B. Anne. / Assessment of requirements for IL-15 and IFN regulatory factors in uterine NK cell differentiation and function during pregnancy. In: Journal of Immunology. 2003 ; Vol. 171, No. 6. pp. 2937-2944.
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