Assessment of tumor response to oxygen challenge using quantitative diffusion MRI in an animal model

Zhongwei Zhang, Qing Yuan, Heling Zhou, Dawen Zhao, Li Li, Jenifer L. Gerberich, Ralph P. Mason

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Purpose To assess tumor response to oxygen challenge using quantitative diffusion magnetic resonance imaging (MRI). Materials and Methods A well-characterized Dunning R3327-AT1 rat prostate cancer line was implanted subcutaneously in the right thigh of male Copenhagen rats (n=8). Diffusion-weighted images (DWI) with multiple b values (0, 25, 50, 100, 150, 200, 300, 500, 1000, 1500 s/mm2) in three orthogonal directions were obtained using a multishot FSE-based Stejskal-Tanner DWI sequence (FSE-DWI) at 4.7T, while rats breathed medical air (21% oxygen) and with 100% oxygen challenge. Stretched-exponential and intravoxel incoherent motion (IVIM) models were used to calculate and compare quantitative diffusion parameters: diffusion heterogeneity index (α), intravoxel distribution of diffusion coefficients (DDC), tissue diffusivity (Dt), pseudo-diffusivity (Dp), and perfusion fraction (f) on a voxel-by-voxel basis. Results A significant increase of α (73.9±4.7% in air vs. 78.1±4.5% in oxygen, P=0.0198) and a significant decrease of f (13.4±3.7% in air vs. 10.4±2.7% in oxygen, P=0.0201) were observed to accompany oxygen challenge. Correlations between f and α during both air and oxygen breathing were found; the correlation coefficients ® were -0.90 and -0.96, respectively. Positive correlations between Dt and DDC with oxygen breathing (r=0.95, P=0.0003), f and DDC with air breathing were also observed (r=0.95, P=0.0004). Conclusion Quantitative diffusion MRI demonstrated changes in tumor perfusion in response to oxygen challenge.

Original languageEnglish (US)
Pages (from-to)1450-1457
Number of pages8
JournalJournal of Magnetic Resonance Imaging
Volume42
Issue number5
DOIs
StatePublished - Nov 1 2015

Fingerprint

Diffusion Magnetic Resonance Imaging
Animal Models
Oxygen
Neoplasms
Air
Respiration
Perfusion
Thigh
Prostatic Neoplasms

Keywords

  • diffusion-weighted imaging
  • hyperoxia
  • intravoxel incoherent motion (IVIM)
  • perfusion
  • stretched-exponential model
  • tumor oxygenation

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Assessment of tumor response to oxygen challenge using quantitative diffusion MRI in an animal model. / Zhang, Zhongwei; Yuan, Qing; Zhou, Heling; Zhao, Dawen; Li, Li; Gerberich, Jenifer L.; Mason, Ralph P.

In: Journal of Magnetic Resonance Imaging, Vol. 42, No. 5, 01.11.2015, p. 1450-1457.

Research output: Contribution to journalArticle

Zhang, Zhongwei ; Yuan, Qing ; Zhou, Heling ; Zhao, Dawen ; Li, Li ; Gerberich, Jenifer L. ; Mason, Ralph P. / Assessment of tumor response to oxygen challenge using quantitative diffusion MRI in an animal model. In: Journal of Magnetic Resonance Imaging. 2015 ; Vol. 42, No. 5. pp. 1450-1457.
@article{be45e086b30c4c60b2148f176123aa54,
title = "Assessment of tumor response to oxygen challenge using quantitative diffusion MRI in an animal model",
abstract = "Purpose To assess tumor response to oxygen challenge using quantitative diffusion magnetic resonance imaging (MRI). Materials and Methods A well-characterized Dunning R3327-AT1 rat prostate cancer line was implanted subcutaneously in the right thigh of male Copenhagen rats (n=8). Diffusion-weighted images (DWI) with multiple b values (0, 25, 50, 100, 150, 200, 300, 500, 1000, 1500 s/mm2) in three orthogonal directions were obtained using a multishot FSE-based Stejskal-Tanner DWI sequence (FSE-DWI) at 4.7T, while rats breathed medical air (21{\%} oxygen) and with 100{\%} oxygen challenge. Stretched-exponential and intravoxel incoherent motion (IVIM) models were used to calculate and compare quantitative diffusion parameters: diffusion heterogeneity index (α), intravoxel distribution of diffusion coefficients (DDC), tissue diffusivity (Dt), pseudo-diffusivity (Dp), and perfusion fraction (f) on a voxel-by-voxel basis. Results A significant increase of α (73.9±4.7{\%} in air vs. 78.1±4.5{\%} in oxygen, P=0.0198) and a significant decrease of f (13.4±3.7{\%} in air vs. 10.4±2.7{\%} in oxygen, P=0.0201) were observed to accompany oxygen challenge. Correlations between f and α during both air and oxygen breathing were found; the correlation coefficients {\circledR} were -0.90 and -0.96, respectively. Positive correlations between Dt and DDC with oxygen breathing (r=0.95, P=0.0003), f and DDC with air breathing were also observed (r=0.95, P=0.0004). Conclusion Quantitative diffusion MRI demonstrated changes in tumor perfusion in response to oxygen challenge.",
keywords = "diffusion-weighted imaging, hyperoxia, intravoxel incoherent motion (IVIM), perfusion, stretched-exponential model, tumor oxygenation",
author = "Zhongwei Zhang and Qing Yuan and Heling Zhou and Dawen Zhao and Li Li and Gerberich, {Jenifer L.} and Mason, {Ralph P.}",
year = "2015",
month = "11",
day = "1",
doi = "10.1002/jmri.24914",
language = "English (US)",
volume = "42",
pages = "1450--1457",
journal = "Journal of Magnetic Resonance Imaging",
issn = "1053-1807",
publisher = "John Wiley and Sons Inc.",
number = "5",

}

TY - JOUR

T1 - Assessment of tumor response to oxygen challenge using quantitative diffusion MRI in an animal model

AU - Zhang, Zhongwei

AU - Yuan, Qing

AU - Zhou, Heling

AU - Zhao, Dawen

AU - Li, Li

AU - Gerberich, Jenifer L.

AU - Mason, Ralph P.

PY - 2015/11/1

Y1 - 2015/11/1

N2 - Purpose To assess tumor response to oxygen challenge using quantitative diffusion magnetic resonance imaging (MRI). Materials and Methods A well-characterized Dunning R3327-AT1 rat prostate cancer line was implanted subcutaneously in the right thigh of male Copenhagen rats (n=8). Diffusion-weighted images (DWI) with multiple b values (0, 25, 50, 100, 150, 200, 300, 500, 1000, 1500 s/mm2) in three orthogonal directions were obtained using a multishot FSE-based Stejskal-Tanner DWI sequence (FSE-DWI) at 4.7T, while rats breathed medical air (21% oxygen) and with 100% oxygen challenge. Stretched-exponential and intravoxel incoherent motion (IVIM) models were used to calculate and compare quantitative diffusion parameters: diffusion heterogeneity index (α), intravoxel distribution of diffusion coefficients (DDC), tissue diffusivity (Dt), pseudo-diffusivity (Dp), and perfusion fraction (f) on a voxel-by-voxel basis. Results A significant increase of α (73.9±4.7% in air vs. 78.1±4.5% in oxygen, P=0.0198) and a significant decrease of f (13.4±3.7% in air vs. 10.4±2.7% in oxygen, P=0.0201) were observed to accompany oxygen challenge. Correlations between f and α during both air and oxygen breathing were found; the correlation coefficients ® were -0.90 and -0.96, respectively. Positive correlations between Dt and DDC with oxygen breathing (r=0.95, P=0.0003), f and DDC with air breathing were also observed (r=0.95, P=0.0004). Conclusion Quantitative diffusion MRI demonstrated changes in tumor perfusion in response to oxygen challenge.

AB - Purpose To assess tumor response to oxygen challenge using quantitative diffusion magnetic resonance imaging (MRI). Materials and Methods A well-characterized Dunning R3327-AT1 rat prostate cancer line was implanted subcutaneously in the right thigh of male Copenhagen rats (n=8). Diffusion-weighted images (DWI) with multiple b values (0, 25, 50, 100, 150, 200, 300, 500, 1000, 1500 s/mm2) in three orthogonal directions were obtained using a multishot FSE-based Stejskal-Tanner DWI sequence (FSE-DWI) at 4.7T, while rats breathed medical air (21% oxygen) and with 100% oxygen challenge. Stretched-exponential and intravoxel incoherent motion (IVIM) models were used to calculate and compare quantitative diffusion parameters: diffusion heterogeneity index (α), intravoxel distribution of diffusion coefficients (DDC), tissue diffusivity (Dt), pseudo-diffusivity (Dp), and perfusion fraction (f) on a voxel-by-voxel basis. Results A significant increase of α (73.9±4.7% in air vs. 78.1±4.5% in oxygen, P=0.0198) and a significant decrease of f (13.4±3.7% in air vs. 10.4±2.7% in oxygen, P=0.0201) were observed to accompany oxygen challenge. Correlations between f and α during both air and oxygen breathing were found; the correlation coefficients ® were -0.90 and -0.96, respectively. Positive correlations between Dt and DDC with oxygen breathing (r=0.95, P=0.0003), f and DDC with air breathing were also observed (r=0.95, P=0.0004). Conclusion Quantitative diffusion MRI demonstrated changes in tumor perfusion in response to oxygen challenge.

KW - diffusion-weighted imaging

KW - hyperoxia

KW - intravoxel incoherent motion (IVIM)

KW - perfusion

KW - stretched-exponential model

KW - tumor oxygenation

UR - http://www.scopus.com/inward/record.url?scp=84945452933&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84945452933&partnerID=8YFLogxK

U2 - 10.1002/jmri.24914

DO - 10.1002/jmri.24914

M3 - Article

C2 - 25866057

AN - SCOPUS:84945452933

VL - 42

SP - 1450

EP - 1457

JO - Journal of Magnetic Resonance Imaging

JF - Journal of Magnetic Resonance Imaging

SN - 1053-1807

IS - 5

ER -