Assignment of determinants recognized by human alloantisera to HLA‐D‐subregion molecules by a lysostrip technique

We have used a lysostripping technique to investigate the molecular relationships of the determinants recognized by human alloantisera and murine monoclonal antibodies reactive with HLA‐D region products. Cytotoxicity mediated by sera that define the specificities DR1, DR2, DR3, DR4, DR7, DRw11, DRw12, DRw13, DRw14, DRw52 and DRw53 was inhibited by anti‐DR but not by anti‐DQ Mabs. In contrast, sera that define DQw1, DQw2 and DQw3 were inhibited by anti‐DQ but not anti‐DR monoclonal antibodies. We found some sera used to define DR specificities that were not inhibited by monoclonal anti‐DR. Cytotoxicity mediated by monoclonal antibodies reactive with the recently described specificities TA10 and MCI was inhibited by anti‐DQ and anti‐DR monoclonal antibodies, respectively. The lysostripping technique is useful for the assignment of specific determinants to HLA‐D subregion molecules.