Association of Cdk-activating kinase subunits with transcription factor TFIIH

Hiroaki Serizawa, Tomi P. Mäkelä, Joan Weliky Conaway, Ronald C. Conaway, Robert A. Weinberg, Richard A. Young

Research output: Contribution to journalArticlepeer-review

303 Scopus citations

Abstract

THE RNA polymerase II large subunit contains an essential carboxy-terminal domain (CTD) believed to be involved in the response to regulators during transcription initiation1–10. The CTD is phosphorylated on a portion of RNA polymerase II molecules in in vivo11, 12 and it can be phosphorylated by the general transcription factor TFIIH in vitro13–15. A highly purified TFIIH from rat liver has been described16; this, like human and yeast TFIIH, contains associated CTD kinase and helicase activities13–18. We report here that two polypeptides of the purified mammalian TFIIH are the MO15/Cdk7 kinase and cyclin H subunits of the Cdk-activating kinase Cak19–21, previously identified as a positive regulator of Cdc2 and Cdk2. TFIIH and Cak preparations are each capable of phosphorylating recombinant CTD and recombi-nant Cdk2 proteins. The presence of Cak in TFIIH indicates that Cak may have roles in transcriptional regulation and in cell-cycle control.

Original languageEnglish (US)
Pages (from-to)280-282
Number of pages3
JournalNature
Volume374
Issue number6519
DOIs
StatePublished - Mar 16 1995
Externally publishedYes

ASJC Scopus subject areas

  • General

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