Association of Endophilin B1 with Cytoplasmic Vesicles

Jinhui Li, Barbara Barylko, John P. Eichorst, Joachim D. Mueller, Joseph P. Albanesi, Yan Chen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Endophilins are SH3- and BAR domain-containing proteins implicated in membrane remodeling and vesicle formation. Endophilins A1 and A2 promote the budding of endocytic vesicles from the plasma membrane, whereas endophilin B1 has been implicated in vesicle budding from intracellular organelles, including the trans-Golgi network and late endosomes. We previously reported that endophilins A1 and A2 exist almost exclusively as soluble dimers in the cytosol. Here, we present results of fluorescence fluctuation spectroscopy analyses indicating that, in contrast, the majority of endophilin B1 is present in multiple copies on small, highly mobile cytoplasmic vesicles. Formation of these vesicles was enhanced by overexpression of wild-type dynamin 2, but suppressed by expression of a catalytically inactive dynamin 2 mutant. Using dual-color heterospecies partition analysis, we identified the epidermal growth factor receptor on endophilin B1 vesicles. Moreover, a proportion of endophilin B1 vesicles also contained caveolin, whereas clathrin was almost undetectable on those vesicles. These results raise the possibility that endophilin B1 participates in dynamin 2-dependent formation of a population of transport vesicles distinct from those generated by A-type endophilins.

Original languageEnglish (US)
Pages (from-to)565-576
Number of pages12
JournalBiophysical Journal
Volume111
Issue number3
DOIs
StatePublished - Aug 9 2016

Fingerprint

Dynamin II
Cytoplasmic Vesicles
Transport Vesicles
varespladib methyl
Caveolins
trans-Golgi Network
Clathrin
src Homology Domains
Endosomes
Fluorescence Spectrometry
Epidermal Growth Factor Receptor
Organelles
Cytosol
Color
Cell Membrane
Membranes
Population
Proteins

ASJC Scopus subject areas

  • Biophysics

Cite this

Association of Endophilin B1 with Cytoplasmic Vesicles. / Li, Jinhui; Barylko, Barbara; Eichorst, John P.; Mueller, Joachim D.; Albanesi, Joseph P.; Chen, Yan.

In: Biophysical Journal, Vol. 111, No. 3, 09.08.2016, p. 565-576.

Research output: Contribution to journalArticle

Li, Jinhui ; Barylko, Barbara ; Eichorst, John P. ; Mueller, Joachim D. ; Albanesi, Joseph P. ; Chen, Yan. / Association of Endophilin B1 with Cytoplasmic Vesicles. In: Biophysical Journal. 2016 ; Vol. 111, No. 3. pp. 565-576.
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