Association of heterogeneous nuclear ribonucleoprotein A1 and C proteins with reiterated AUUUA sequences

B. Jonell Hamilton, Eszter Nagy, James S. Malter, Bradley A. Arrick, William F C Rigby

Research output: Contribution to journalArticle

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Abstract

Post-transcriptional regulatory mechanisms have been shown to play a major role in gene expression in eukaryotic cells. The presence of a reiterated pentamer (AUUUA) in the 3′-untranslated region (UTR) of mRNAs encoding lymphokines, cytokines, transcription factors, and proto-oncogenes has been shown to be associated with rapid turnover and translation attenuation. Cytoplasmic proteins (70, 50, 43, 36, and 25 kDa) capable of specifically binding to RNAs containing these AU-rich sequences were identified in human peripheral blood T lymphocytes. Levels of the 36-kDa protein were markedly increased following transcriptional, but not translational inhibition, a feature recently reported for hnRNP A1, a protein of comparable mass. Antibodies directed against heterogeneous nuclear ribonucleoproteins (hnRNPs) A1 and C immuno-precipitated 36- and 43-kDa proteins that had bound the AUUUA-rich region contained in the 3′-UTR of granulocyte-macrophage colony-stimulating factor mRNA. Recombinant hnRNP A1 was shown to preferentially bind to RNAs containing AUUUA sequences in a specific manner, and displayed comparable patterns to the 36-kDa AU-specific binding proteins following partial proteolysis. These data identify for the first time hnRNP A1 and C as cytoplasmic proteins in human lymphocytes that are capable of specifically associating with reiterated AUUUA sequences present in the 3′-UTR of labile mRNAs. As such, they may play a role as trans-acting factors in the modulation of cytoplasmic mRNA turnover and translation, in addition to their previously characterized roles as pre-mRNA binding proteins involved in nuclear mRNA processing.

Original languageEnglish (US)
Pages (from-to)8881-8887
Number of pages7
JournalJournal of Biological Chemistry
Volume268
Issue number12
StatePublished - Apr 25 1993

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Heterogeneous-Nuclear Ribonucleoprotein Group C
Heterogeneous-Nuclear Ribonucleoproteins
Protein C
3' Untranslated Regions
Messenger RNA
Carrier Proteins
Proteins
RNA
Trans-Activators
Proto-Oncogenes
Lymphokines
RNA Precursors
Proteolysis
Protein Biosynthesis
Eukaryotic Cells
Granulocyte-Macrophage Colony-Stimulating Factor
T-cells
Lymphocytes
Transcription Factors
Gene expression

ASJC Scopus subject areas

  • Biochemistry

Cite this

Association of heterogeneous nuclear ribonucleoprotein A1 and C proteins with reiterated AUUUA sequences. / Hamilton, B. Jonell; Nagy, Eszter; Malter, James S.; Arrick, Bradley A.; Rigby, William F C.

In: Journal of Biological Chemistry, Vol. 268, No. 12, 25.04.1993, p. 8881-8887.

Research output: Contribution to journalArticle

Hamilton, B. Jonell ; Nagy, Eszter ; Malter, James S. ; Arrick, Bradley A. ; Rigby, William F C. / Association of heterogeneous nuclear ribonucleoprotein A1 and C proteins with reiterated AUUUA sequences. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 12. pp. 8881-8887.
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