Associations of anti-β2-glycoprotein I autoantibodies with HLA class II alleles in three ethnic groups

Frank C. Arnett, Perumal Thiagarajan, Chul Ahn, John D. Reveille

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Objective. To determine any HLA associations with anti-β2- glycoprotein I (anti-β2GPI) antibodies in a large, retrospectively studied, multiethnic group of 262 patients with primary antiphospholipid antibody syndrome (APS), systemic lupus erythematosus (SLE), or another connective tissue disease. Methods. Anti-β2GPI antibodies were detected in sera using an enzyme-linked immunosorbent assay. HLA class II alleles (DRB1, DQA1, and DQB1) were determined by DNA oligotyping. Results. The HLA-DQB1*0302 (DQ8) allele, typically carried on HLA-DR4 haplotypes, was associated with anti- β2GPI when compared with both anti-β2GPI-negative SLE patients and ethnically matched normal controls, especially in Mexican Americans and, to a lesser extent, in whites. Similarly, when ethnic groups were combined, HLA- DQB1*0302, as well as HLA-DQB1*03 alleles overall (DQB1*0301,*0302, and *0303), were strongly correlated with anti-β2GPI antibodies. The HLA-DR6 (DR13) haplotype DRB1*1302; DQB1*0604/5 was also significantly increased, primarily in blacks. HLA-DR7 was not significantly increased in any of these 3 ethnic groups, and HLA-DR53 (DRB4*0101) was increased in Mexican Americans only. Conclusion. Certain HLA class II haplotypes genetically influence the expression of antibodies to β2GPI, an important autoimmune response in the APS, but there are variations in HLA associations among different ethnic groups.

Original languageEnglish (US)
Pages (from-to)268-274
Number of pages7
JournalArthritis and Rheumatism
Volume42
Issue number2
DOIs
StatePublished - Feb 1999

Fingerprint

Ethnic Groups
Autoantibodies
Haplotypes
Glycoproteins
Antiphospholipid Syndrome
Alleles
Systemic Lupus Erythematosus
HLA-DRB4 Chains
HLA-DR6 Antigen
Antibodies
HLA-DR7 Antigen
HLA-DR4 Antigen
Connective Tissue Diseases
Autoimmunity
Anti-Idiotypic Antibodies
Enzyme-Linked Immunosorbent Assay
DNA
Serum
HLA-DQB1 antigen

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Associations of anti-β2-glycoprotein I autoantibodies with HLA class II alleles in three ethnic groups. / Arnett, Frank C.; Thiagarajan, Perumal; Ahn, Chul; Reveille, John D.

In: Arthritis and Rheumatism, Vol. 42, No. 2, 02.1999, p. 268-274.

Research output: Contribution to journalArticle

Arnett, Frank C. ; Thiagarajan, Perumal ; Ahn, Chul ; Reveille, John D. / Associations of anti-β2-glycoprotein I autoantibodies with HLA class II alleles in three ethnic groups. In: Arthritis and Rheumatism. 1999 ; Vol. 42, No. 2. pp. 268-274.
@article{f63f48b4a36e462788ab8d59664fe5f7,
title = "Associations of anti-β2-glycoprotein I autoantibodies with HLA class II alleles in three ethnic groups",
abstract = "Objective. To determine any HLA associations with anti-β2- glycoprotein I (anti-β2GPI) antibodies in a large, retrospectively studied, multiethnic group of 262 patients with primary antiphospholipid antibody syndrome (APS), systemic lupus erythematosus (SLE), or another connective tissue disease. Methods. Anti-β2GPI antibodies were detected in sera using an enzyme-linked immunosorbent assay. HLA class II alleles (DRB1, DQA1, and DQB1) were determined by DNA oligotyping. Results. The HLA-DQB1*0302 (DQ8) allele, typically carried on HLA-DR4 haplotypes, was associated with anti- β2GPI when compared with both anti-β2GPI-negative SLE patients and ethnically matched normal controls, especially in Mexican Americans and, to a lesser extent, in whites. Similarly, when ethnic groups were combined, HLA- DQB1*0302, as well as HLA-DQB1*03 alleles overall (DQB1*0301,*0302, and *0303), were strongly correlated with anti-β2GPI antibodies. The HLA-DR6 (DR13) haplotype DRB1*1302; DQB1*0604/5 was also significantly increased, primarily in blacks. HLA-DR7 was not significantly increased in any of these 3 ethnic groups, and HLA-DR53 (DRB4*0101) was increased in Mexican Americans only. Conclusion. Certain HLA class II haplotypes genetically influence the expression of antibodies to β2GPI, an important autoimmune response in the APS, but there are variations in HLA associations among different ethnic groups.",
author = "Arnett, {Frank C.} and Perumal Thiagarajan and Chul Ahn and Reveille, {John D.}",
year = "1999",
month = "2",
doi = "10.1002/1529-0131(199902)42:2<268::AID-ANR8>3.0.CO;2-K",
language = "English (US)",
volume = "42",
pages = "268--274",
journal = "Arthritis and Rheumatology",
issn = "2326-5191",
publisher = "John Wiley and Sons Ltd",
number = "2",

}

TY - JOUR

T1 - Associations of anti-β2-glycoprotein I autoantibodies with HLA class II alleles in three ethnic groups

AU - Arnett, Frank C.

AU - Thiagarajan, Perumal

AU - Ahn, Chul

AU - Reveille, John D.

PY - 1999/2

Y1 - 1999/2

N2 - Objective. To determine any HLA associations with anti-β2- glycoprotein I (anti-β2GPI) antibodies in a large, retrospectively studied, multiethnic group of 262 patients with primary antiphospholipid antibody syndrome (APS), systemic lupus erythematosus (SLE), or another connective tissue disease. Methods. Anti-β2GPI antibodies were detected in sera using an enzyme-linked immunosorbent assay. HLA class II alleles (DRB1, DQA1, and DQB1) were determined by DNA oligotyping. Results. The HLA-DQB1*0302 (DQ8) allele, typically carried on HLA-DR4 haplotypes, was associated with anti- β2GPI when compared with both anti-β2GPI-negative SLE patients and ethnically matched normal controls, especially in Mexican Americans and, to a lesser extent, in whites. Similarly, when ethnic groups were combined, HLA- DQB1*0302, as well as HLA-DQB1*03 alleles overall (DQB1*0301,*0302, and *0303), were strongly correlated with anti-β2GPI antibodies. The HLA-DR6 (DR13) haplotype DRB1*1302; DQB1*0604/5 was also significantly increased, primarily in blacks. HLA-DR7 was not significantly increased in any of these 3 ethnic groups, and HLA-DR53 (DRB4*0101) was increased in Mexican Americans only. Conclusion. Certain HLA class II haplotypes genetically influence the expression of antibodies to β2GPI, an important autoimmune response in the APS, but there are variations in HLA associations among different ethnic groups.

AB - Objective. To determine any HLA associations with anti-β2- glycoprotein I (anti-β2GPI) antibodies in a large, retrospectively studied, multiethnic group of 262 patients with primary antiphospholipid antibody syndrome (APS), systemic lupus erythematosus (SLE), or another connective tissue disease. Methods. Anti-β2GPI antibodies were detected in sera using an enzyme-linked immunosorbent assay. HLA class II alleles (DRB1, DQA1, and DQB1) were determined by DNA oligotyping. Results. The HLA-DQB1*0302 (DQ8) allele, typically carried on HLA-DR4 haplotypes, was associated with anti- β2GPI when compared with both anti-β2GPI-negative SLE patients and ethnically matched normal controls, especially in Mexican Americans and, to a lesser extent, in whites. Similarly, when ethnic groups were combined, HLA- DQB1*0302, as well as HLA-DQB1*03 alleles overall (DQB1*0301,*0302, and *0303), were strongly correlated with anti-β2GPI antibodies. The HLA-DR6 (DR13) haplotype DRB1*1302; DQB1*0604/5 was also significantly increased, primarily in blacks. HLA-DR7 was not significantly increased in any of these 3 ethnic groups, and HLA-DR53 (DRB4*0101) was increased in Mexican Americans only. Conclusion. Certain HLA class II haplotypes genetically influence the expression of antibodies to β2GPI, an important autoimmune response in the APS, but there are variations in HLA associations among different ethnic groups.

UR - http://www.scopus.com/inward/record.url?scp=0032588621&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032588621&partnerID=8YFLogxK

U2 - 10.1002/1529-0131(199902)42:2<268::AID-ANR8>3.0.CO;2-K

DO - 10.1002/1529-0131(199902)42:2<268::AID-ANR8>3.0.CO;2-K

M3 - Article

VL - 42

SP - 268

EP - 274

JO - Arthritis and Rheumatology

JF - Arthritis and Rheumatology

SN - 2326-5191

IS - 2

ER -