Associations of MHC class II alleles with insulin-dependent diabetes mellitus (IDDM) in patients from North India

Rajni Rani, Ajay Sood, Ana M. Lazaro, Peter Stastny

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Thirty-four insulin-dependent diabetes mellitus (IDDM) patients from North India were studied with respect to their HLA class. It alleles including those of the DRB1, DQA1, DQB1 and DPB1 loci, using the polymerase chain reaction (PCR) and hybridization with sequence-specific oligonucleotide probes (SSOP). They were compared with the class II alleles of 94 normal adult controls from the same ethnic background. The results show a statistically significant increase of DRB1*03011 (p < 0.00001), DQB1*0201 (p < 0.007), DQA1*0501 (0.0027) and DPB1*2601 (p < 0.0042) in patients compared to controls. DR*04 was not significantly increased. However, homozygosity for DRB1*03011 was increased more than expected. DRB1*1501 and *1502 did not show a significant decrease in the patients. However, DRB1*0701 was decreased significantly, but this difference did not remain significant when the p value was corrected for the number of alleles tested. Similarly, DPB1*2601 was increased significantly in the patients but did not remain significant after p was corrected for the number of alleles tested. However, DPB1*2601 was increased, and remained significant after correction, in patients not having HLA-DR3. We also studied the possible role of aspartic acid at codon 57 of the DQ beta chain in protection against development of diabetes, and arginine at codon 52 of the DQ alpha chain in susceptibility. We observed an increase in non-Asp57 alleles in DQ beta and Arg52 in DQ alpha in the patients, however, this effect seems to be due to the fact that the most prevalent haplotype in diabetic patients: DRB1*03011-DQA1*0501- DQB1*0201, has DQB1 and DQA1 alleles which carry non-Asp57 and Arg52, respectively.

Original languageEnglish (US)
Pages (from-to)524-531
Number of pages8
JournalHuman Immunology
Volume60
Issue number6
DOIs
StatePublished - Jun 1999

Fingerprint

Type 1 Diabetes Mellitus
India
Alleles
Codon
HLA-DR3 Antigen
Oligonucleotide Probes
Aspartic Acid
Haplotypes
Arginine
Polymerase Chain Reaction

Keywords

  • IDDM
  • Polymerase chain reaction (PCR)
  • Sequence specific oligonucleotide probes (SSOP)

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Associations of MHC class II alleles with insulin-dependent diabetes mellitus (IDDM) in patients from North India. / Rani, Rajni; Sood, Ajay; Lazaro, Ana M.; Stastny, Peter.

In: Human Immunology, Vol. 60, No. 6, 06.1999, p. 524-531.

Research output: Contribution to journalArticle

Rani, Rajni ; Sood, Ajay ; Lazaro, Ana M. ; Stastny, Peter. / Associations of MHC class II alleles with insulin-dependent diabetes mellitus (IDDM) in patients from North India. In: Human Immunology. 1999 ; Vol. 60, No. 6. pp. 524-531.
@article{d00dcba233124203920ab3f325ee4112,
title = "Associations of MHC class II alleles with insulin-dependent diabetes mellitus (IDDM) in patients from North India",
abstract = "Thirty-four insulin-dependent diabetes mellitus (IDDM) patients from North India were studied with respect to their HLA class. It alleles including those of the DRB1, DQA1, DQB1 and DPB1 loci, using the polymerase chain reaction (PCR) and hybridization with sequence-specific oligonucleotide probes (SSOP). They were compared with the class II alleles of 94 normal adult controls from the same ethnic background. The results show a statistically significant increase of DRB1*03011 (p < 0.00001), DQB1*0201 (p < 0.007), DQA1*0501 (0.0027) and DPB1*2601 (p < 0.0042) in patients compared to controls. DR*04 was not significantly increased. However, homozygosity for DRB1*03011 was increased more than expected. DRB1*1501 and *1502 did not show a significant decrease in the patients. However, DRB1*0701 was decreased significantly, but this difference did not remain significant when the p value was corrected for the number of alleles tested. Similarly, DPB1*2601 was increased significantly in the patients but did not remain significant after p was corrected for the number of alleles tested. However, DPB1*2601 was increased, and remained significant after correction, in patients not having HLA-DR3. We also studied the possible role of aspartic acid at codon 57 of the DQ beta chain in protection against development of diabetes, and arginine at codon 52 of the DQ alpha chain in susceptibility. We observed an increase in non-Asp57 alleles in DQ beta and Arg52 in DQ alpha in the patients, however, this effect seems to be due to the fact that the most prevalent haplotype in diabetic patients: DRB1*03011-DQA1*0501- DQB1*0201, has DQB1 and DQA1 alleles which carry non-Asp57 and Arg52, respectively.",
keywords = "IDDM, Polymerase chain reaction (PCR), Sequence specific oligonucleotide probes (SSOP)",
author = "Rajni Rani and Ajay Sood and Lazaro, {Ana M.} and Peter Stastny",
year = "1999",
month = "6",
doi = "10.1016/S0198-8859(99)00032-4",
language = "English (US)",
volume = "60",
pages = "524--531",
journal = "Human Immunology",
issn = "0198-8859",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Associations of MHC class II alleles with insulin-dependent diabetes mellitus (IDDM) in patients from North India

AU - Rani, Rajni

AU - Sood, Ajay

AU - Lazaro, Ana M.

AU - Stastny, Peter

PY - 1999/6

Y1 - 1999/6

N2 - Thirty-four insulin-dependent diabetes mellitus (IDDM) patients from North India were studied with respect to their HLA class. It alleles including those of the DRB1, DQA1, DQB1 and DPB1 loci, using the polymerase chain reaction (PCR) and hybridization with sequence-specific oligonucleotide probes (SSOP). They were compared with the class II alleles of 94 normal adult controls from the same ethnic background. The results show a statistically significant increase of DRB1*03011 (p < 0.00001), DQB1*0201 (p < 0.007), DQA1*0501 (0.0027) and DPB1*2601 (p < 0.0042) in patients compared to controls. DR*04 was not significantly increased. However, homozygosity for DRB1*03011 was increased more than expected. DRB1*1501 and *1502 did not show a significant decrease in the patients. However, DRB1*0701 was decreased significantly, but this difference did not remain significant when the p value was corrected for the number of alleles tested. Similarly, DPB1*2601 was increased significantly in the patients but did not remain significant after p was corrected for the number of alleles tested. However, DPB1*2601 was increased, and remained significant after correction, in patients not having HLA-DR3. We also studied the possible role of aspartic acid at codon 57 of the DQ beta chain in protection against development of diabetes, and arginine at codon 52 of the DQ alpha chain in susceptibility. We observed an increase in non-Asp57 alleles in DQ beta and Arg52 in DQ alpha in the patients, however, this effect seems to be due to the fact that the most prevalent haplotype in diabetic patients: DRB1*03011-DQA1*0501- DQB1*0201, has DQB1 and DQA1 alleles which carry non-Asp57 and Arg52, respectively.

AB - Thirty-four insulin-dependent diabetes mellitus (IDDM) patients from North India were studied with respect to their HLA class. It alleles including those of the DRB1, DQA1, DQB1 and DPB1 loci, using the polymerase chain reaction (PCR) and hybridization with sequence-specific oligonucleotide probes (SSOP). They were compared with the class II alleles of 94 normal adult controls from the same ethnic background. The results show a statistically significant increase of DRB1*03011 (p < 0.00001), DQB1*0201 (p < 0.007), DQA1*0501 (0.0027) and DPB1*2601 (p < 0.0042) in patients compared to controls. DR*04 was not significantly increased. However, homozygosity for DRB1*03011 was increased more than expected. DRB1*1501 and *1502 did not show a significant decrease in the patients. However, DRB1*0701 was decreased significantly, but this difference did not remain significant when the p value was corrected for the number of alleles tested. Similarly, DPB1*2601 was increased significantly in the patients but did not remain significant after p was corrected for the number of alleles tested. However, DPB1*2601 was increased, and remained significant after correction, in patients not having HLA-DR3. We also studied the possible role of aspartic acid at codon 57 of the DQ beta chain in protection against development of diabetes, and arginine at codon 52 of the DQ alpha chain in susceptibility. We observed an increase in non-Asp57 alleles in DQ beta and Arg52 in DQ alpha in the patients, however, this effect seems to be due to the fact that the most prevalent haplotype in diabetic patients: DRB1*03011-DQA1*0501- DQB1*0201, has DQB1 and DQA1 alleles which carry non-Asp57 and Arg52, respectively.

KW - IDDM

KW - Polymerase chain reaction (PCR)

KW - Sequence specific oligonucleotide probes (SSOP)

UR - http://www.scopus.com/inward/record.url?scp=0033024150&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033024150&partnerID=8YFLogxK

U2 - 10.1016/S0198-8859(99)00032-4

DO - 10.1016/S0198-8859(99)00032-4

M3 - Article

C2 - 10408802

AN - SCOPUS:0033024150

VL - 60

SP - 524

EP - 531

JO - Human Immunology

JF - Human Immunology

SN - 0198-8859

IS - 6

ER -