Asymmetric Sensory-Motor Regeneration of Transected Peripheral Nerves Using Molecular Guidance Cues

Sanjay Anand, Vidhi Desai, Nesreen Alsmadi, Aswini Kanneganti, Dianna Huyen Tram Nguyen, Martin Tran, Lokesh Patil, Srikanth Vasudevan, Cancan Xu, Yi Hong, Jonathan Cheng, Edward Keefer, Mario I. Romero-Ortega

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Neural interfaces are designed to decode motor intent and evoke sensory precepts in amputees. In peripheral nerves, recording movement intent is challenging because motor axons are only a small fraction compared to sensory fibers and are heterogeneously mixed particularly at proximal levels. We previously reported that pain and myelinated axons regenerating through a Y-shaped nerve guide with sealed ends, can be modulated by luminar release of nerve growth factor (NGF) and neurotrophin-3 (NT-3), respectively. Here, we evaluate the differential potency of NGF, glial cell line-derived neurotrophic factor (GDNF), brain-derived neurotrophic factor (BDNF), pleiotrophin (PTN), and NT-3 in asymmetrically guiding the regeneration of sensory and motor neurons. We report that, in the absence of distal target organs, molecular guidance cues can mediate the growth of electrically conductive fascicles with normal microanatomy. Compared to Y-tube compartments with bovine serum albumin (BSA), GDNF and NGF increased the motor and sensory axon content, respectively. In addition, the sensory to motor ratio was significantly increased by PTN (12.7:1) when compared to a BDNF + GDNF choice. The differential content of motor and sensory axons modulated by selective guidance cues may provide a strategy to better define axon types in peripheral nerve interfaces.

Original languageEnglish (US)
Article number14323
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

Fingerprint

Peripheral Nerves
Cues
Axons
Regeneration
Glial Cell Line-Derived Neurotrophic Factor
Nerve Growth Factor
Neurotrophin 3
Brain-Derived Neurotrophic Factor
Amputees
Motor Neurons
Sensory Receptor Cells
Bovine Serum Albumin
Pain
Growth
pleiotrophin

ASJC Scopus subject areas

  • General

Cite this

Anand, S., Desai, V., Alsmadi, N., Kanneganti, A., Nguyen, D. H. T., Tran, M., ... Romero-Ortega, M. I. (2017). Asymmetric Sensory-Motor Regeneration of Transected Peripheral Nerves Using Molecular Guidance Cues. Scientific Reports, 7(1), [14323]. https://doi.org/10.1038/s41598-017-14331-x

Asymmetric Sensory-Motor Regeneration of Transected Peripheral Nerves Using Molecular Guidance Cues. / Anand, Sanjay; Desai, Vidhi; Alsmadi, Nesreen; Kanneganti, Aswini; Nguyen, Dianna Huyen Tram; Tran, Martin; Patil, Lokesh; Vasudevan, Srikanth; Xu, Cancan; Hong, Yi; Cheng, Jonathan; Keefer, Edward; Romero-Ortega, Mario I.

In: Scientific Reports, Vol. 7, No. 1, 14323, 01.12.2017.

Research output: Contribution to journalArticle

Anand, S, Desai, V, Alsmadi, N, Kanneganti, A, Nguyen, DHT, Tran, M, Patil, L, Vasudevan, S, Xu, C, Hong, Y, Cheng, J, Keefer, E & Romero-Ortega, MI 2017, 'Asymmetric Sensory-Motor Regeneration of Transected Peripheral Nerves Using Molecular Guidance Cues', Scientific Reports, vol. 7, no. 1, 14323. https://doi.org/10.1038/s41598-017-14331-x
Anand, Sanjay ; Desai, Vidhi ; Alsmadi, Nesreen ; Kanneganti, Aswini ; Nguyen, Dianna Huyen Tram ; Tran, Martin ; Patil, Lokesh ; Vasudevan, Srikanth ; Xu, Cancan ; Hong, Yi ; Cheng, Jonathan ; Keefer, Edward ; Romero-Ortega, Mario I. / Asymmetric Sensory-Motor Regeneration of Transected Peripheral Nerves Using Molecular Guidance Cues. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
@article{26fcd26f3d05456f934af14ba02c3f88,
title = "Asymmetric Sensory-Motor Regeneration of Transected Peripheral Nerves Using Molecular Guidance Cues",
abstract = "Neural interfaces are designed to decode motor intent and evoke sensory precepts in amputees. In peripheral nerves, recording movement intent is challenging because motor axons are only a small fraction compared to sensory fibers and are heterogeneously mixed particularly at proximal levels. We previously reported that pain and myelinated axons regenerating through a Y-shaped nerve guide with sealed ends, can be modulated by luminar release of nerve growth factor (NGF) and neurotrophin-3 (NT-3), respectively. Here, we evaluate the differential potency of NGF, glial cell line-derived neurotrophic factor (GDNF), brain-derived neurotrophic factor (BDNF), pleiotrophin (PTN), and NT-3 in asymmetrically guiding the regeneration of sensory and motor neurons. We report that, in the absence of distal target organs, molecular guidance cues can mediate the growth of electrically conductive fascicles with normal microanatomy. Compared to Y-tube compartments with bovine serum albumin (BSA), GDNF and NGF increased the motor and sensory axon content, respectively. In addition, the sensory to motor ratio was significantly increased by PTN (12.7:1) when compared to a BDNF + GDNF choice. The differential content of motor and sensory axons modulated by selective guidance cues may provide a strategy to better define axon types in peripheral nerve interfaces.",
author = "Sanjay Anand and Vidhi Desai and Nesreen Alsmadi and Aswini Kanneganti and Nguyen, {Dianna Huyen Tram} and Martin Tran and Lokesh Patil and Srikanth Vasudevan and Cancan Xu and Yi Hong and Jonathan Cheng and Edward Keefer and Romero-Ortega, {Mario I.}",
year = "2017",
month = "12",
day = "1",
doi = "10.1038/s41598-017-14331-x",
language = "English (US)",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Asymmetric Sensory-Motor Regeneration of Transected Peripheral Nerves Using Molecular Guidance Cues

AU - Anand, Sanjay

AU - Desai, Vidhi

AU - Alsmadi, Nesreen

AU - Kanneganti, Aswini

AU - Nguyen, Dianna Huyen Tram

AU - Tran, Martin

AU - Patil, Lokesh

AU - Vasudevan, Srikanth

AU - Xu, Cancan

AU - Hong, Yi

AU - Cheng, Jonathan

AU - Keefer, Edward

AU - Romero-Ortega, Mario I.

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Neural interfaces are designed to decode motor intent and evoke sensory precepts in amputees. In peripheral nerves, recording movement intent is challenging because motor axons are only a small fraction compared to sensory fibers and are heterogeneously mixed particularly at proximal levels. We previously reported that pain and myelinated axons regenerating through a Y-shaped nerve guide with sealed ends, can be modulated by luminar release of nerve growth factor (NGF) and neurotrophin-3 (NT-3), respectively. Here, we evaluate the differential potency of NGF, glial cell line-derived neurotrophic factor (GDNF), brain-derived neurotrophic factor (BDNF), pleiotrophin (PTN), and NT-3 in asymmetrically guiding the regeneration of sensory and motor neurons. We report that, in the absence of distal target organs, molecular guidance cues can mediate the growth of electrically conductive fascicles with normal microanatomy. Compared to Y-tube compartments with bovine serum albumin (BSA), GDNF and NGF increased the motor and sensory axon content, respectively. In addition, the sensory to motor ratio was significantly increased by PTN (12.7:1) when compared to a BDNF + GDNF choice. The differential content of motor and sensory axons modulated by selective guidance cues may provide a strategy to better define axon types in peripheral nerve interfaces.

AB - Neural interfaces are designed to decode motor intent and evoke sensory precepts in amputees. In peripheral nerves, recording movement intent is challenging because motor axons are only a small fraction compared to sensory fibers and are heterogeneously mixed particularly at proximal levels. We previously reported that pain and myelinated axons regenerating through a Y-shaped nerve guide with sealed ends, can be modulated by luminar release of nerve growth factor (NGF) and neurotrophin-3 (NT-3), respectively. Here, we evaluate the differential potency of NGF, glial cell line-derived neurotrophic factor (GDNF), brain-derived neurotrophic factor (BDNF), pleiotrophin (PTN), and NT-3 in asymmetrically guiding the regeneration of sensory and motor neurons. We report that, in the absence of distal target organs, molecular guidance cues can mediate the growth of electrically conductive fascicles with normal microanatomy. Compared to Y-tube compartments with bovine serum albumin (BSA), GDNF and NGF increased the motor and sensory axon content, respectively. In addition, the sensory to motor ratio was significantly increased by PTN (12.7:1) when compared to a BDNF + GDNF choice. The differential content of motor and sensory axons modulated by selective guidance cues may provide a strategy to better define axon types in peripheral nerve interfaces.

UR - http://www.scopus.com/inward/record.url?scp=85032586724&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85032586724&partnerID=8YFLogxK

U2 - 10.1038/s41598-017-14331-x

DO - 10.1038/s41598-017-14331-x

M3 - Article

C2 - 29085079

AN - SCOPUS:85032586724

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 14323

ER -