Abstract
Background: ATPase subunits mediate 26 S proteasome assembly and function. Results: Defective ATP binding by some ATPase subunits inhibits proteasome assembly, and proteasomes harboring an ATP binding-defective subunit are impaired for proteolysis, substrate-stimulated gating, and ATPase activity. Conclusion: The proteasome requires normal ATP binding for assembly and function. Significance: Protein substrates promote their own degradation by inducing ATP-dependent proteasome functions.
Original language | English (US) |
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Pages (from-to) | 3334-3345 |
Number of pages | 12 |
Journal | Journal of Biological Chemistry |
Volume | 288 |
Issue number | 5 |
DOIs | |
State | Published - Feb 1 2013 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology