Abstract
Background: ATPase subunits mediate 26 S proteasome assembly and function. Results: Defective ATP binding by some ATPase subunits inhibits proteasome assembly, and proteasomes harboring an ATP binding-defective subunit are impaired for proteolysis, substrate-stimulated gating, and ATPase activity. Conclusion: The proteasome requires normal ATP binding for assembly and function. Significance: Protein substrates promote their own degradation by inducing ATP-dependent proteasome functions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3334-3345 |
| Number of pages | 12 |
| Journal | Journal of Biological Chemistry |
| Volume | 288 |
| Issue number | 5 |
| DOIs | |
| State | Published - Feb 1 2013 |
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ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Molecular Biology
Cite this
ATP binding by proteasomal ATPases regulates cellular assembly and substrate-induced functions of the 26 S proteasome. / Kim, Young Chan; Li, Xiaohua; Thompson, David; Demartino, George N.
In: Journal of Biological Chemistry, Vol. 288, No. 5, 01.02.2013, p. 3334-3345.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - ATP binding by proteasomal ATPases regulates cellular assembly and substrate-induced functions of the 26 S proteasome
AU - Kim, Young Chan
AU - Li, Xiaohua
AU - Thompson, David
AU - Demartino, George N.
PY - 2013/2/1
Y1 - 2013/2/1
N2 - Background: ATPase subunits mediate 26 S proteasome assembly and function. Results: Defective ATP binding by some ATPase subunits inhibits proteasome assembly, and proteasomes harboring an ATP binding-defective subunit are impaired for proteolysis, substrate-stimulated gating, and ATPase activity. Conclusion: The proteasome requires normal ATP binding for assembly and function. Significance: Protein substrates promote their own degradation by inducing ATP-dependent proteasome functions.
AB - Background: ATPase subunits mediate 26 S proteasome assembly and function. Results: Defective ATP binding by some ATPase subunits inhibits proteasome assembly, and proteasomes harboring an ATP binding-defective subunit are impaired for proteolysis, substrate-stimulated gating, and ATPase activity. Conclusion: The proteasome requires normal ATP binding for assembly and function. Significance: Protein substrates promote their own degradation by inducing ATP-dependent proteasome functions.
UR - http://www.scopus.com/inward/record.url?scp=84873321806&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84873321806&partnerID=8YFLogxK
U2 - 10.1074/jbc.M112.424788
DO - 10.1074/jbc.M112.424788
M3 - Article
VL - 288
SP - 3334
EP - 3345
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 5
ER -