ATP binding by proteasomal ATPases regulates cellular assembly and substrate-induced functions of the 26 S proteasome

Young Chan Kim; Xiaohua Li; David Thompson; George N. Demartino

doi:10.1074/jbc.M112.424788

ATP binding by proteasomal ATPases regulates cellular assembly and substrate-induced functions of the 26 S proteasome

Young Chan Kim, Xiaohua Li, David Thompson, George N. Demartino

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Background: ATPase subunits mediate 26 S proteasome assembly and function. Results: Defective ATP binding by some ATPase subunits inhibits proteasome assembly, and proteasomes harboring an ATP binding-defective subunit are impaired for proteolysis, substrate-stimulated gating, and ATPase activity. Conclusion: The proteasome requires normal ATP binding for assembly and function. Significance: Protein substrates promote their own degradation by inducing ATP-dependent proteasome functions.

Original language	English (US)
Pages (from-to)	3334-3345
Number of pages	12
Journal	Journal of Biological Chemistry
Volume	288
Issue number	5
DOIs	https://doi.org/10.1074/jbc.M112.424788
State	Published - Feb 1 2013

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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abstract = "Background: ATPase subunits mediate 26 S proteasome assembly and function. Results: Defective ATP binding by some ATPase subunits inhibits proteasome assembly, and proteasomes harboring an ATP binding-defective subunit are impaired for proteolysis, substrate-stimulated gating, and ATPase activity. Conclusion: The proteasome requires normal ATP binding for assembly and function. Significance: Protein substrates promote their own degradation by inducing ATP-dependent proteasome functions.",

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AU - Li, Xiaohua

AU - Thompson, David

AU - Demartino, George N.

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N2 - Background: ATPase subunits mediate 26 S proteasome assembly and function. Results: Defective ATP binding by some ATPase subunits inhibits proteasome assembly, and proteasomes harboring an ATP binding-defective subunit are impaired for proteolysis, substrate-stimulated gating, and ATPase activity. Conclusion: The proteasome requires normal ATP binding for assembly and function. Significance: Protein substrates promote their own degradation by inducing ATP-dependent proteasome functions.

AB - Background: ATPase subunits mediate 26 S proteasome assembly and function. Results: Defective ATP binding by some ATPase subunits inhibits proteasome assembly, and proteasomes harboring an ATP binding-defective subunit are impaired for proteolysis, substrate-stimulated gating, and ATPase activity. Conclusion: The proteasome requires normal ATP binding for assembly and function. Significance: Protein substrates promote their own degradation by inducing ATP-dependent proteasome functions.

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ATP binding by proteasomal ATPases regulates cellular assembly and substrate-induced functions of the 26 S proteasome

Abstract

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