Atrial natriuretic factor receptors in cultured renomedullary interstitial cells

B. M A Fontoura, D. R. Nussenzveig, K. M. Pelton, T. Maack

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Abstract

To gain further insight on the cell types that may mediate the effects of atrial natriuretic factor (ANF) in the renal medulla, we determined the distribution and function of biological (B) and clearance (C) receptors of ANF in renomedullary interstitial cells (RMIC). Studies were performed in the 3rd-17th passages of RMIC obtained from a primary culture of the rat renal medulla. Electron microscopy of the cultured cells showed the typical morphological features of RMIC 'in vivo', including prominent lipid droplets. RMIC have a very high density of high-affinity specific binding sites of ANF-(1-28) [23,000 sites/cell; dissociation constant (K(d)) = 50 pM]. There was only minimal binding of C-ANF-l(4-23) (< 2,500 sites/cell), a specific ligand of C-ANF receptors. ANF-(1-28) markedly increased guanosine 3',5'-cyclic monophosphate (cGMP) from 1.3 ± 0.3 to 106 ± 22 pmol cGMP/106 cells [50% effective dosage (ED50) = 1.2 nM]. The effect of ANF-(1-28) on cGMP was nearly additive to that of sodium nitroprusside and was not potentiated or antagonized by C-ANF-(4-23). The density of guanylate cyclase-coupled B-ANF receptors and the ANF-induced increase in cGMP in RMIC are higher than those reported to date in other target cells. This suggests that RMIC may mediate some of the known effects f ANF in the renal medulla.

Original languageEnglish (US)
Pages (from-to)C692-C699
JournalAmerican Journal of Physiology - Cell Physiology
Volume258
Issue number4 27-4
StatePublished - May 15 1990

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Keywords

  • atrial natriuretic peptide
  • atrial peptide analogues
  • biological receptor of atrial natriuretic factor
  • cell culture
  • clearance receptor of atrial natriuretic factor
  • guanosine 3',5'-cyclic monophosphate
  • ultrastructure of cultured renomedullary interstitial cells

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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