TY - JOUR
T1 - Atrial natriuretic factor receptors in cultured renomedullary interstitial cells
AU - Fontoura, B. M A
AU - Nussenzveig, D. R.
AU - Pelton, K. M.
AU - Maack, T.
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 1990
Y1 - 1990
N2 - To gain further insight on the cell types that may mediate the effects of atrial natriuretic factor (ANF) in the renal medulla, we determined the distribution and function of biological (B) and clearance (C) receptors of ANF in renomedullary interstitial cells (RMIC). Studies were performed in the 3rd-17th passages of RMIC obtained from a primary culture of the rat renal medulla. Electron microscopy of the cultured cells showed the typical morphological features of RMIC 'in vivo', including prominent lipid droplets. RMIC have a very high density of high-affinity specific binding sites of ANF-(1-28) [23,000 sites/cell; dissociation constant (K(d)) = 50 pM]. There was only minimal binding of C-ANF-l(4-23) (< 2,500 sites/cell), a specific ligand of C-ANF receptors. ANF-(1-28) markedly increased guanosine 3',5'-cyclic monophosphate (cGMP) from 1.3 ± 0.3 to 106 ± 22 pmol cGMP/106 cells [50% effective dosage (ED50) = 1.2 nM]. The effect of ANF-(1-28) on cGMP was nearly additive to that of sodium nitroprusside and was not potentiated or antagonized by C-ANF-(4-23). The density of guanylate cyclase-coupled B-ANF receptors and the ANF-induced increase in cGMP in RMIC are higher than those reported to date in other target cells. This suggests that RMIC may mediate some of the known effects f ANF in the renal medulla.
AB - To gain further insight on the cell types that may mediate the effects of atrial natriuretic factor (ANF) in the renal medulla, we determined the distribution and function of biological (B) and clearance (C) receptors of ANF in renomedullary interstitial cells (RMIC). Studies were performed in the 3rd-17th passages of RMIC obtained from a primary culture of the rat renal medulla. Electron microscopy of the cultured cells showed the typical morphological features of RMIC 'in vivo', including prominent lipid droplets. RMIC have a very high density of high-affinity specific binding sites of ANF-(1-28) [23,000 sites/cell; dissociation constant (K(d)) = 50 pM]. There was only minimal binding of C-ANF-l(4-23) (< 2,500 sites/cell), a specific ligand of C-ANF receptors. ANF-(1-28) markedly increased guanosine 3',5'-cyclic monophosphate (cGMP) from 1.3 ± 0.3 to 106 ± 22 pmol cGMP/106 cells [50% effective dosage (ED50) = 1.2 nM]. The effect of ANF-(1-28) on cGMP was nearly additive to that of sodium nitroprusside and was not potentiated or antagonized by C-ANF-(4-23). The density of guanylate cyclase-coupled B-ANF receptors and the ANF-induced increase in cGMP in RMIC are higher than those reported to date in other target cells. This suggests that RMIC may mediate some of the known effects f ANF in the renal medulla.
KW - atrial natriuretic peptide
KW - atrial peptide analogues
KW - biological receptor of atrial natriuretic factor
KW - cell culture
KW - clearance receptor of atrial natriuretic factor
KW - guanosine 3',5'-cyclic monophosphate
KW - ultrastructure of cultured renomedullary interstitial cells
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U2 - 10.1152/ajpcell.1990.258.4.c692
DO - 10.1152/ajpcell.1990.258.4.c692
M3 - Article
C2 - 2159215
AN - SCOPUS:0025361247
VL - 258
SP - C692-C699
JO - American Journal of Physiology - Heart and Circulatory Physiology
JF - American Journal of Physiology - Heart and Circulatory Physiology
SN - 0363-6135
IS - 4 27-4
ER -