ATXR5 and ATXR6 are H3K27 monomethyltransferases required for chromatin structure and gene silencing

Yannick Jacob, Suhua Feng, Chantal A. LeBlanc, Yana V. Bernatavichute, Hume Stroud, Shawn Cokus, Lianna M. Johnson, Matteo Pellegrini, Steven E. Jacobsen, Scott D. Michaels

Research output: Contribution to journalArticlepeer-review

Abstract

Constitutive heterochromatin in Arabidopsis thaliana is marked by repressive chromatin modifications, including DNA methylation, histone H3 dimethylation at Lys9 (H3K9me2) and monomethylation at Lys27 (H3K27me1). The enzymes catalyzing DNA methylation and H3K9me2 have been identified; alterations in these proteins lead to reactivation of silenced heterochromatic elements. The enzymes responsible for heterochromatic H3K27me1, in contrast, remain unknown. Here we show that the divergent SET-domain proteins ARABIDOPSIS TRITHORAX-RELATED PROTEIN 5 (ATXR5) and ATXR6 have H3K27 monomethyltransferase activity, and atxr5 atxr6 double mutants have reduced H3K27me1 in vivo and show partial heterochromatin decondensation. Mutations in atxr5 and atxr6 also lead to transcriptional activation of repressed heterochromatic elements. Notably, H3K9me2 and DNA methylation are unaffected in double mutants. These results indicate that ATXR5 and ATXR6 form a new class of H3K27 methyltransferases and that H3K27me1 represents a previously uncharacterized pathway required for transcriptional repression in Arabidopsis.

Original languageEnglish (US)
Pages (from-to)763-768
Number of pages6
JournalNature Structural and Molecular Biology
Volume16
Issue number7
DOIs
StatePublished - Jul 2009
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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