Atypical enteropathogenic Escherichia coli that contains functional locus of enterocyte effacement genes can be attaching-and-effacing negative in cultured epithelial cells

Sérgio P D Rocha, Cecilia M. Abe, Vanessa Sperandio, Silvia Y. Bando, Waldir P. Elias

Research output: Contribution to journalArticle

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Abstract

Enteropathogenic Escherichia coli (EPEC) induces a characteristic histopathology on enterocytes known as the attaching-and-effacing (A/E) lesion, which is triggered by proteins encoded by the locus of enterocyte effacement (LEE). EPEC is currently classified as typical EPEC (tEPEC) and atypical EPEC (aEPEC), based on the presence or absence of the EPEC adherence factor plasmid, respectively. Here we analyzed the LEE regions of three aEPEC strains displaying the localized adherence-like (LAL), aggregative adherence (AA), and diffuse adherence (DA) patterns on HEp-2 cells as well as one nonadherent (NA) strain. The adherence characteristics and the ability to induce A/E lesions were investigated with HeLa, Caco-2, T84, and HT29 cells. The adherence patterns and fluorescent actin staining (FAS) assay results were reproducible with all cell lines. The LEE region was structurally intact and functional in all strains regardless of their inability to cause A/E lesions. An EspFU-expressing plasmid (pKC471) was introduced into all strains, demonstrating no influence of this protein on either the adherence patterns or the capacity to cause A/E of the adherent strains. However, the NA strain harboring pKC471 expressed the LAL pattern and was able to induce A/E lesions on HeLa cells. Our data indicate that FAS-negative aEPEC strains are potentially able to induce A/E in vivo, emphasizing the concern about this test for the determination of aEPEC virulence. Also, the presence of EspFU was sufficient to provide an adherent phenotype for a nonadherent aEPEC strain via the direct or indirect activation of the LEE4 and LEE5 operons.

Original languageEnglish (US)
Pages (from-to)1833-1841
Number of pages9
JournalInfection and Immunity
Volume79
Issue number5
DOIs
StatePublished - May 2011

Fingerprint

Enteropathogenic Escherichia coli
Enterocytes
Cultured Cells
Epithelial Cells
Genes
Actins
Plasmids
Negative Staining
HT29 Cells
Caco-2 Cells
Operon
HeLa Cells
Virulence
Proteins
Staining and Labeling
Phenotype
Cell Line

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Atypical enteropathogenic Escherichia coli that contains functional locus of enterocyte effacement genes can be attaching-and-effacing negative in cultured epithelial cells. / Rocha, Sérgio P D; Abe, Cecilia M.; Sperandio, Vanessa; Bando, Silvia Y.; Elias, Waldir P.

In: Infection and Immunity, Vol. 79, No. 5, 05.2011, p. 1833-1841.

Research output: Contribution to journalArticle

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