Autoantibodies to small heat shock proteins in glaucoma

Gülgün Tezel, Gail M. Seigel, Martin B. Wax

Research output: Contribution to journalArticle

171 Citations (Scopus)

Abstract

PURPOSE. To identify the low-molecular-weight retinal proteins that are the targets of serum autoantibodies in patients with glaucoma and to study the ability of these antibodies to induce retinal apoptosis. METHODS. Serum immunoreactivity against retinal proteins was examined in age-matched groups of 60 patients with normal-pressure glaucoma, 36 patients with high-pressure glaucoma, and a control group of 20 healthy subjects, by means of western blot analysis and enzyme-linked immunosorbent assay. The specificity of the immunoreactivity to small heat shock proteins; including α-crystallins and hsp27, was tested by immunoprecipitation of these proteins in retinal fractions. The direct effects of antibodies specific to small heat shock proteins were then studied in isolated intact human retina (ex vivo) and cultured rat retinal cells (in vitro) by immunocytochemistry and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) technique in conjunction with fluorescence microscopy and confocal imaging. RESULTS. Serum immunoreactivity against retinal proteins with low molecular weight in patients with glaucoma was to small heat shock proteins, including α-crystallins and hsp27. In addition, patients with normal pressure glaucoma had a higher titer of autoantibodies to small heat shock proteins than did age-matched patients with high-pressure glaucoma or control subjects. It was observed that when antibodies against small heat shock proteins were applied directly to retina tissue or cells, they could trigger cell death through an apoptotic mechanism. CONCLUSIONS. These findings suggest that increased titers of circulating antibodies against retinal small heat shock proteins may have pathogenic significance in some patients with glaucomatous optic neuropathy.

Original languageEnglish (US)
Pages (from-to)2277-2287
Number of pages11
JournalInvestigative Ophthalmology and Visual Science
Volume39
Issue number12
StatePublished - Nov 1998

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Small Heat-Shock Proteins
Glaucoma
Autoantibodies
Pressure
Crystallins
Antibodies
Retina
Molecular Weight
Optic Nerve Diseases
Proteins
DNA Nucleotidylexotransferase
Serum
Fluorescence Microscopy
Immunoprecipitation
Blood Proteins
Healthy Volunteers
Cell Death
Research Design
Age Groups
Western Blotting

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Tezel, G., Seigel, G. M., & Wax, M. B. (1998). Autoantibodies to small heat shock proteins in glaucoma. Investigative Ophthalmology and Visual Science, 39(12), 2277-2287.

Autoantibodies to small heat shock proteins in glaucoma. / Tezel, Gülgün; Seigel, Gail M.; Wax, Martin B.

In: Investigative Ophthalmology and Visual Science, Vol. 39, No. 12, 11.1998, p. 2277-2287.

Research output: Contribution to journalArticle

Tezel, G, Seigel, GM & Wax, MB 1998, 'Autoantibodies to small heat shock proteins in glaucoma', Investigative Ophthalmology and Visual Science, vol. 39, no. 12, pp. 2277-2287.
Tezel, Gülgün ; Seigel, Gail M. ; Wax, Martin B. / Autoantibodies to small heat shock proteins in glaucoma. In: Investigative Ophthalmology and Visual Science. 1998 ; Vol. 39, No. 12. pp. 2277-2287.
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