Autoantibodies to the δ-opioid receptor function as opioid agonists and display immunomodulatory activity

Parvathi Ranganathan; Hao Chen; Miranda K. Adelman; Samuel F. Schluter

doi:10.1016/j.jneuroim.2009.10.007

Autoantibodies to the δ-opioid receptor function as opioid agonists and display immunomodulatory activity

Parvathi Ranganathan, Hao Chen, Miranda K. Adelman, Samuel F. Schluter

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

In this report, we show that affinity purified human anti-delta opioid receptor (DOR) autoantibodies from IVIG are specific to DOR and possess agonistic properties displayed by their ability to dramatically decrease forskolin stimulated cAMP accumulation. Anti-DOR autoantibody also caused phosphorylation of the opioid receptor. Anti-DOR autoantibody treatment showed a significant reduction in CXCR4 gene expression as well as surface protein expression. In contrast, anti-DOR autoantibody treatment significantly upregulated CCR5 gene and protein expression. The presence of anti-DOR autoantibodies in IVIG and their potent immunomodulatory activity is further evidence to support the cross-talk between the neuroendocrine and immune systems.

Original language	English (US)
Pages (from-to)	65-73
Number of pages	9
Journal	Journal of Neuroimmunology
Volume	217
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2009.10.007
State	Published - Dec 10 2009
Externally published	Yes

Keywords

Autoantibodies
Immunomodulation
Opioid receptors

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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10.1016/j.jneuroim.2009.10.007

Cite this

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title = "Autoantibodies to the δ-opioid receptor function as opioid agonists and display immunomodulatory activity",

abstract = "In this report, we show that affinity purified human anti-delta opioid receptor (DOR) autoantibodies from IVIG are specific to DOR and possess agonistic properties displayed by their ability to dramatically decrease forskolin stimulated cAMP accumulation. Anti-DOR autoantibody also caused phosphorylation of the opioid receptor. Anti-DOR autoantibody treatment showed a significant reduction in CXCR4 gene expression as well as surface protein expression. In contrast, anti-DOR autoantibody treatment significantly upregulated CCR5 gene and protein expression. The presence of anti-DOR autoantibodies in IVIG and their potent immunomodulatory activity is further evidence to support the cross-talk between the neuroendocrine and immune systems.",

keywords = "Autoantibodies, Immunomodulation, Opioid receptors",

author = "Parvathi Ranganathan and Hao Chen and Adelman, {Miranda K.} and Schluter, {Samuel F.}",

note = "Funding Information: This work was supported by a grant from the Arizona Biomedical Research Commission Contract Number 0711 and National Institute of Health Grant NIDA 330726 . We thank Carol Haussler for the maintenance of the CHO DOR cell line.",

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AU - Ranganathan, Parvathi

AU - Chen, Hao

AU - Adelman, Miranda K.

AU - Schluter, Samuel F.

N1 - Funding Information: This work was supported by a grant from the Arizona Biomedical Research Commission Contract Number 0711 and National Institute of Health Grant NIDA 330726 . We thank Carol Haussler for the maintenance of the CHO DOR cell line.

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N2 - In this report, we show that affinity purified human anti-delta opioid receptor (DOR) autoantibodies from IVIG are specific to DOR and possess agonistic properties displayed by their ability to dramatically decrease forskolin stimulated cAMP accumulation. Anti-DOR autoantibody also caused phosphorylation of the opioid receptor. Anti-DOR autoantibody treatment showed a significant reduction in CXCR4 gene expression as well as surface protein expression. In contrast, anti-DOR autoantibody treatment significantly upregulated CCR5 gene and protein expression. The presence of anti-DOR autoantibodies in IVIG and their potent immunomodulatory activity is further evidence to support the cross-talk between the neuroendocrine and immune systems.

AB - In this report, we show that affinity purified human anti-delta opioid receptor (DOR) autoantibodies from IVIG are specific to DOR and possess agonistic properties displayed by their ability to dramatically decrease forskolin stimulated cAMP accumulation. Anti-DOR autoantibody also caused phosphorylation of the opioid receptor. Anti-DOR autoantibody treatment showed a significant reduction in CXCR4 gene expression as well as surface protein expression. In contrast, anti-DOR autoantibody treatment significantly upregulated CCR5 gene and protein expression. The presence of anti-DOR autoantibodies in IVIG and their potent immunomodulatory activity is further evidence to support the cross-talk between the neuroendocrine and immune systems.

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KW - Immunomodulation

KW - Opioid receptors

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Autoantibodies to the δ-opioid receptor function as opioid agonists and display immunomodulatory activity

Abstract

Keywords

ASJC Scopus subject areas

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