Autoantibodies to the GLUT-2 glucose transporter of β cells in insulin-dependent diabetes mellitus of recent onset

Lindsey R. Inman, Chris T. Mcallister, Ling Chen, Steven Hughes, Christopher B. Newgard, John R. Kettman, Roger H Unger, John H. Johnson

Research output: Contribution to journalArticle

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Abstract

Purified immunoglobulin G (IgG) from the serum of patients with insulin-dependent diabetes mellitus (IDDM) of recent onset inhibits high-Km uptake of 3-O-methyl-β-D-glucose by rat pancreatic islets. To determine if the inhibition is the result of antibodies against GLUT-2, the high-Km glucose transporter of β cells, we incubated IDDM sera with rat islet cells and with AtT-20ins cells transfected to express GLUT-2. IDDM sera inhibited glucose uptake in islet cells and in GLUT-2-expressing AtT-20ins cells but not in AtT-20ins cells transfected to express the low-Km isoform, GLUT-1. In 24 of 30 (77%) patients with newly diagnosed IDDM, IgG binding as measured by immunofluorescence and flow cytometry of the cells transfected to express GLUT-2 was >2 standard deviations from the mean of the nondiabetic population; 29 of 31 (96%) of nondiabetic children were negative (P < 0.0001). Increased IgG binding could be removed by absorption with GLUT-2-expressing cells but not with GLUT-1-expressing cells. We conclude that most patients with IDDM of recent onset have autoantibodies to GLUT-2.

Original languageEnglish (US)
Pages (from-to)1281-1284
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number4
StatePublished - Feb 15 1993

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Facilitative Glucose Transport Proteins
Type 1 Diabetes Mellitus
Autoantibodies
Islets of Langerhans
Immunoglobulin G
Serum
3-O-Methylglucose
Fluorescent Antibody Technique
Flow Cytometry
Protein Isoforms
Glucose
Antibodies
Population

Keywords

  • Flow cytometry

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Inman, L. R., Mcallister, C. T., Chen, L., Hughes, S., Newgard, C. B., Kettman, J. R., ... Johnson, J. H. (1993). Autoantibodies to the GLUT-2 glucose transporter of β cells in insulin-dependent diabetes mellitus of recent onset. Proceedings of the National Academy of Sciences of the United States of America, 90(4), 1281-1284.

Autoantibodies to the GLUT-2 glucose transporter of β cells in insulin-dependent diabetes mellitus of recent onset. / Inman, Lindsey R.; Mcallister, Chris T.; Chen, Ling; Hughes, Steven; Newgard, Christopher B.; Kettman, John R.; Unger, Roger H; Johnson, John H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 4, 15.02.1993, p. 1281-1284.

Research output: Contribution to journalArticle

Inman, Lindsey R. ; Mcallister, Chris T. ; Chen, Ling ; Hughes, Steven ; Newgard, Christopher B. ; Kettman, John R. ; Unger, Roger H ; Johnson, John H. / Autoantibodies to the GLUT-2 glucose transporter of β cells in insulin-dependent diabetes mellitus of recent onset. In: Proceedings of the National Academy of Sciences of the United States of America. 1993 ; Vol. 90, No. 4. pp. 1281-1284.
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