Autoantibodies to the GLUT-2 glucose transporter of β cells in insulin- dependent diabetes mellitus of recent onset

Lindsey R. Inman, Chris T. Mcallister, Ling Chen, Steven Hughes, Christopher B. Newgard, John R. Kettman, Roger H Unger, John H. Johnson

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

Purified immunoglobulin G (IgG) from the serum of patients with insulin- dependent diabetes mellitus (IDDM) of recent onset inhibits high-K(m) uptake of 3-O-methyl-β-D-glucose by rat pancreatic islets. To determine if the inhibition is the result of antibodies against GLUT-2, the high-K(m) glucose transporter of β cells, we incubated IDDM sera with rat islet cells and with AtT-20(ins) cells transfected to express GLUT-2. IDDM sera inhibited glucose uptake in islet cells and in GLUT-2-expressing AtT-20(ins) cells but not in AtT-20(ins) cells transfected to express the low-K(m) isoform, GLUT-1. In 24 of 30 (77%) patients with newly diagnosed IDDM, IgG binding as measured by immunofluorescence and flow cytometry of the cells transfected to express GLUT-2 was >2 standard deviations from the mean of the nondiabetic population; 29 of 31 (96%) of nondiabetic children were negative (P < 0.0001). Increased IgG binding could be removed by absorption with GLUT-2- expressing cells but not with GLUT-1-expressing cells. We conclude that most patients with IDDM of recent onset have autoantibodies to GLUT-2.

Original languageEnglish (US)
Pages (from-to)1281-1284
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number4
DOIs
StatePublished - Mar 1 1993

Keywords

  • flow cytometry

ASJC Scopus subject areas

  • General

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