Autonomous replication activity of a human mitochondrial DNA sequence inserted into genomic DNA

T. O. Nielsen, M. A. Piatyszek, J. W. Shay, C. E. Pearson, M. Zannis-Hadjopoulus, G. B. Price

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Mitochondrial DNA (mtDNA) fragments can be found inserted into nuclear DNA and may contribute to cancer and aging. A HeLaTG-cell nuclear transcript was shown to include human cytochrome oxidase subunit 3 (coxIII) mtDNA fused to c-myc sequences. Independently, a coxIII-containing cDNA was discovered to replicate autonomously in HeLa cells. We show that the HeLaTG mtDNA insertion has autonomous replication activity when transfected into HeLa cells and in a mammalian in vitro replication system. Replication in vitro starts within the fusion cDNA sequence. Insertion of an element which could function as a replication origin may affect cellular DNA replication and proliferation.

Original languageEnglish (US)
Pages (from-to)1003-1008
Number of pages6
JournalInternational Journal of Oncology
Volume5
Issue number5
StatePublished - 1994

Fingerprint

DNA Replication
Mitochondrial DNA
Human Activities
HeLa Cells
DNA
Complementary DNA
Replication Origin
Electron Transport Complex IV
Neoplasms
In Vitro Techniques

Keywords

  • Cytochrome oxidase III
  • DNA replication
  • In vitro replication
  • Insertional mutagenesis
  • Mitochondrial DNA
  • Replication origin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Nielsen, T. O., Piatyszek, M. A., Shay, J. W., Pearson, C. E., Zannis-Hadjopoulus, M., & Price, G. B. (1994). Autonomous replication activity of a human mitochondrial DNA sequence inserted into genomic DNA. International Journal of Oncology, 5(5), 1003-1008.

Autonomous replication activity of a human mitochondrial DNA sequence inserted into genomic DNA. / Nielsen, T. O.; Piatyszek, M. A.; Shay, J. W.; Pearson, C. E.; Zannis-Hadjopoulus, M.; Price, G. B.

In: International Journal of Oncology, Vol. 5, No. 5, 1994, p. 1003-1008.

Research output: Contribution to journalArticle

Nielsen, TO, Piatyszek, MA, Shay, JW, Pearson, CE, Zannis-Hadjopoulus, M & Price, GB 1994, 'Autonomous replication activity of a human mitochondrial DNA sequence inserted into genomic DNA', International Journal of Oncology, vol. 5, no. 5, pp. 1003-1008.
Nielsen TO, Piatyszek MA, Shay JW, Pearson CE, Zannis-Hadjopoulus M, Price GB. Autonomous replication activity of a human mitochondrial DNA sequence inserted into genomic DNA. International Journal of Oncology. 1994;5(5):1003-1008.
Nielsen, T. O. ; Piatyszek, M. A. ; Shay, J. W. ; Pearson, C. E. ; Zannis-Hadjopoulus, M. ; Price, G. B. / Autonomous replication activity of a human mitochondrial DNA sequence inserted into genomic DNA. In: International Journal of Oncology. 1994 ; Vol. 5, No. 5. pp. 1003-1008.
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