Autonomous replication activity of a human mitochondrial DNA sequence inserted into genomic DNA

T. O. Nielsen, M. A. Piatyszek, J. W. Shay, C. E. Pearson, M. Zannis-Hadjopoulus, G. B. Price

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Mitochondrial DNA (mtDNA) fragments can be found inserted into nuclear DNA and may contribute to cancer and aging. A HeLaTG-cell nuclear transcript was shown to include human cytochrome oxidase subunit 3 (coxIII) mtDNA fused to c-myc sequences. Independently, a coxIII-containing cDNA was discovered to replicate autonomously in HeLa cells. We show that the HeLaTG mtDNA insertion has autonomous replication activity when transfected into HeLa cells and in a mammalian in vitro replication system. Replication in vitro starts within the fusion cDNA sequence. Insertion of an element which could function as a replication origin may affect cellular DNA replication and proliferation.

Original languageEnglish (US)
Pages (from-to)1003-1008
Number of pages6
JournalInternational journal of oncology
Volume5
Issue number5
DOIs
StatePublished - Jan 1 1994

Keywords

  • Cytochrome oxidase III
  • DNA replication
  • In vitro replication
  • Insertional mutagenesis
  • Mitochondrial DNA
  • Replication origin

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Nielsen, T. O., Piatyszek, M. A., Shay, J. W., Pearson, C. E., Zannis-Hadjopoulus, M., & Price, G. B. (1994). Autonomous replication activity of a human mitochondrial DNA sequence inserted into genomic DNA. International journal of oncology, 5(5), 1003-1008. https://doi.org/10.3892/ijo.5.5.1003