Autophagy promotes primary ciliogenesis by removing OFD1 from centriolar satellites

Zaiming Tang, Mary Grace Lin, Timothy Richard Stowe, She Chen, Muyuan Zhu, Tim Stearns, Brunella Franco, Qing Zhong

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

The primary cilium is a microtubule-based organelle that functions in sensory and signalling pathways. Defects in ciliogenesis can lead to a group of genetic syndromes known as ciliopathies. However, the regulatory mechanisms of primary ciliogenesis in normal and cancer cells are incompletely understood. Here we demonstrate that autophagic degradation of a ciliopathy protein, OFD1 (oral-facial-digital syndrome 1), at centriolar satellites promotes primary cilium biogenesis. Autophagy is a catabolic pathway in which cytosol, damaged organelles and protein aggregates are engulfed in autophagosomes and delivered to lysosomes for destruction. We show that the population of OFD1 at the centriolar satellites is rapidly degraded by autophagy upon serum starvation. In autophagy-deficient Atg5 or Atg3 null mouse embryonic fibroblasts, OFD1 accumulates at centriolar satellites, leading to fewer and shorter primary cilia and a defective recruitment of BBS4 (Bardet-Biedl syndrome 4) to cilia. These defects are fully rescued by OFD1 partial knockdown that reduces the population of OFD1 at centriolar satellites. More strikingly, OFD1 depletion at centriolar satellites promotes cilia formation in both cycling cells and transformed breast cancer MCF7 cells that normally do not form cilia. This work reveals that removal of OFD1 by autophagy at centriolar satellites represents a general mechanism to promote ciliogenesis in mammalian cells. These findings define a newly recognized role of autophagy in organelle biogenesis.

Original languageEnglish (US)
Pages (from-to)254-257
Number of pages4
JournalNature
Volume502
Issue number7470
DOIs
StatePublished - 2013

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Orofaciodigital Syndromes
Autophagy
Cilia
Organelles
MCF-7 Cells
Organelle Biogenesis
Starvation
Lysosomes
Microtubules
Cytosol
Population
Fibroblasts
Breast Neoplasms

ASJC Scopus subject areas

  • General

Cite this

Tang, Z., Lin, M. G., Stowe, T. R., Chen, S., Zhu, M., Stearns, T., ... Zhong, Q. (2013). Autophagy promotes primary ciliogenesis by removing OFD1 from centriolar satellites. Nature, 502(7470), 254-257. https://doi.org/10.1038/nature12606

Autophagy promotes primary ciliogenesis by removing OFD1 from centriolar satellites. / Tang, Zaiming; Lin, Mary Grace; Stowe, Timothy Richard; Chen, She; Zhu, Muyuan; Stearns, Tim; Franco, Brunella; Zhong, Qing.

In: Nature, Vol. 502, No. 7470, 2013, p. 254-257.

Research output: Contribution to journalArticle

Tang, Z, Lin, MG, Stowe, TR, Chen, S, Zhu, M, Stearns, T, Franco, B & Zhong, Q 2013, 'Autophagy promotes primary ciliogenesis by removing OFD1 from centriolar satellites', Nature, vol. 502, no. 7470, pp. 254-257. https://doi.org/10.1038/nature12606
Tang Z, Lin MG, Stowe TR, Chen S, Zhu M, Stearns T et al. Autophagy promotes primary ciliogenesis by removing OFD1 from centriolar satellites. Nature. 2013;502(7470):254-257. https://doi.org/10.1038/nature12606
Tang, Zaiming ; Lin, Mary Grace ; Stowe, Timothy Richard ; Chen, She ; Zhu, Muyuan ; Stearns, Tim ; Franco, Brunella ; Zhong, Qing. / Autophagy promotes primary ciliogenesis by removing OFD1 from centriolar satellites. In: Nature. 2013 ; Vol. 502, No. 7470. pp. 254-257.
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