Autophosphorylation at serine 1981 stabilizes ATM at DNA damage sites

Sairei So, Anthony J. Davis, David J. Chen

Research output: Contribution to journalArticlepeer-review

125 Scopus citations

Abstract

Ataxia telangiectasia mutated (ATM) plays a critical role in the cellular response to DNA damage. In response to DNA double-strand breaks (DSBs), ATM is autophosphorylated at serine 1981. Although this autophosphorylation is widely considered a sign of ATM activation, it is still not clear if autophosphorylation is required for ATM functions including localization to DSBs and activation of ATM kinase activity. In this study, we show that localization of ATM to DSBs is differentially regulated with the initial localization requiring the MRE11-RAD50-NBS1 complex and sustained retention requiring autophosphorylation of ATM at serine 1981. Autophosphorylated ATM interacts with MDC1 and the latter is required for the prolonged association of ATM to DSBs. Ablation of ATM autophosphorylation or knockdown of MDC1 protein affects the ability of ATM to phosphorylate downstream substrates and confer radioresistance. Together, these data suggest that autophosphorylation at serine 1981 stabilizes ATM at the sites of DSBs, and this is required for a proper DNA damage response.

Original languageEnglish (US)
Pages (from-to)977-990
Number of pages14
JournalJournal of Cell Biology
Volume187
Issue number7
DOIs
StatePublished - Dec 28 2009

ASJC Scopus subject areas

  • Cell Biology

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