Autophosphorylation of αCaMKII is not a general requirement for NMDA receptor-dependent LTP in the adult mouse

Sam F. Cooke, Jianqun Wu, Florian Plattner, Michael Errington, Michael Rowan, Marco Peters, Ayumi Hirano, Karl D. Bradshaw, Roger Anwyl, Timothy V P Bliss, K. Peter Giese

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Abstract

Autophosphorylation of α-Ca2+/calmodulin kinase II (αCaMKII) at Thr286 is thought to be a general effector mechanism for sustaining transcription-independent long-term potentiation (LTP) at pathways where LTP is NMDA receptor-dependent. We have compared LTP at two such hippocampal pathways in mutant mice with a disabling point mutation at the Thr286 autophosphorylation site. We find that autophosphorylation of αCaMKII is essential for induction of LTP at Schaffer commissural-CA1 synapses in vivo, but is not required for LTP that can be sustained over days at medial perforant path-granule cell synapses in awake mice. At these latter synapses LTP is supported by cyclic AMP-dependent signalling in the absence of αCaMKII signalling. Thus, the autophosphorylation of αCaMKII is not a general requirement for NMDA receptor-dependent LTP in the adult mouse.

Original languageEnglish (US)
Pages (from-to)805-818
Number of pages14
JournalJournal of Physiology
Volume574
Issue number3
DOIs
StatePublished - Aug 2006

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Calcium-Calmodulin-Dependent Protein Kinases
Long-Term Potentiation
N-Methyl-D-Aspartate Receptors
Synapses
Perforant Pathway
Point Mutation
Cyclic AMP

ASJC Scopus subject areas

  • Physiology

Cite this

Cooke, S. F., Wu, J., Plattner, F., Errington, M., Rowan, M., Peters, M., ... Giese, K. P. (2006). Autophosphorylation of αCaMKII is not a general requirement for NMDA receptor-dependent LTP in the adult mouse. Journal of Physiology, 574(3), 805-818. https://doi.org/10.1113/jphysiol.2006.111559

Autophosphorylation of αCaMKII is not a general requirement for NMDA receptor-dependent LTP in the adult mouse. / Cooke, Sam F.; Wu, Jianqun; Plattner, Florian; Errington, Michael; Rowan, Michael; Peters, Marco; Hirano, Ayumi; Bradshaw, Karl D.; Anwyl, Roger; Bliss, Timothy V P; Giese, K. Peter.

In: Journal of Physiology, Vol. 574, No. 3, 08.2006, p. 805-818.

Research output: Contribution to journalArticle

Cooke, SF, Wu, J, Plattner, F, Errington, M, Rowan, M, Peters, M, Hirano, A, Bradshaw, KD, Anwyl, R, Bliss, TVP & Giese, KP 2006, 'Autophosphorylation of αCaMKII is not a general requirement for NMDA receptor-dependent LTP in the adult mouse', Journal of Physiology, vol. 574, no. 3, pp. 805-818. https://doi.org/10.1113/jphysiol.2006.111559
Cooke, Sam F. ; Wu, Jianqun ; Plattner, Florian ; Errington, Michael ; Rowan, Michael ; Peters, Marco ; Hirano, Ayumi ; Bradshaw, Karl D. ; Anwyl, Roger ; Bliss, Timothy V P ; Giese, K. Peter. / Autophosphorylation of αCaMKII is not a general requirement for NMDA receptor-dependent LTP in the adult mouse. In: Journal of Physiology. 2006 ; Vol. 574, No. 3. pp. 805-818.
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