Autoradiographic distribution in rat tissues of binding sites for endothelin: A neuropeptide?

C. Koseki, M. Imai, Y. Hirata, Masashi Yanagisawa, T. Masaki

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Abstract

Endothelin (ET) is a potent and long-acting vasoconstrictor peptide consisting of 21 amino acids and recently isolated from a medium of cultured porcine endothelial cells. To determine the possible sites of ET action, we have conducted autoradiography and receptor binding assays with 125I-labeled ET in rat tissues. The displaceable binding sites of the ligand were widely distributed, not only in the arteries and heart but also in various other organs, e.g., brain, kidney, lung, adrenal gland, and intestine. The systemically injected ET did not cross the blood-brain barrier, whereas the ligand, applied in vitro, was mainly located in the hypothalamic and thalamic areas, lateral ventricular region, subfornical organ, globus pallidus, and caudate putamen. Both membrane preparations from the brain stem including diencephalon and from the heart ventricle had similar, specific, and high-affinity binding sites for 125I-ET. We suggest that ET is involved in the regulation of a large variety of organ functions and may also act as a neuropeptide.

Original languageEnglish (US)
Pages (from-to)25/4
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume256
Issue number4
StatePublished - Jan 1 1989

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ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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